GrainGenes Sequence Report: Ta.27117.6.S1_x

UniGene title 'Transcribed locus, moderately similar to NP_851200.1 ACA8 (AUTOINHIBITED CA2+ -ATPASE, ISOFORM 8); calcium-transporting ATPase/ calmodulin binding [Arabidopsis thaliana]'

Keyword

EST

Germplasm

Chinese Spring

Species

Triticum aestivum

Cultivar

Chinese Spring

Clone Library

Wheat salt-stressed crown cDNA library

Tissue

Crown

Developmental Stage

Adult plant

Data Source

genbank	Release 135, Apr 15 2003
genbank	Updated Nov 2006

Title

WHE2106_F08_K16ZS Wheat salt-stressed crown cDNA library Triticum aestivum cDNA clone WHE2106_F08_K16, mRNA sequence.

Other Name

WHE2106_F08_K16ZS [ wEST-mySQL (obsolete) ]

Strain

lab_host E. coli SOLR

DNA Library

TA038E1X

Clone

WHE2106_F08_K16

Probe

BF474831

Remark

DB_xref: taxon:4565

Feature: source: mol_type = 'mRNA';

Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.

Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown under hydroponic conditions at UC Davis, salt stressed for 12 hours, and for 7 days, then dissected and frozen (Akhunov in J Dvorak Lab). Total RNA and poly(A) RNA were prepared from crown tissue, equal portions of RNA were pooled from the two treatments, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab at the University of California, Riverside (Akhunov, Chin, Choi, Close, Fenton, Kianian, Otto, Simons, Zhang). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

DNA

ccgccgccgccgccgcgacgcgaaaggagagcgaggccgactccgccgcc
gcgatgagggccaagtggaagaagaagaggatgaggaggctcaagaggaa
gcgccgaaagatgaggcagcgatccaagtaggcgccttggagcagcggaa
gcatcatcctacatccaggctgccaggcaccgagggatgacggcatggtc
tagtgattcctggcgtgtttgctgctatggctgtggaagctgtttaacct
gtgttatgtccaatttagctgtttaattctagtctggttatgttccaaga
cagagttttatgtaatgtggggattatatgtgtttgtgactgaattttat
gcacctattaattttgcggcgtctcatcttttttgtgatgcgatgatgct
a


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