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GrainGenes Sequence Report: BF482744

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Sequence
BF482744
Tracefile
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External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
wEST map position
BF482744
DB Remark
Locus Source: Triticum aestivum (bread wheat)
Keyword
EST
Germplasm
Chinese Spring
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
5BL
5DL
Clone Library
Wheat pre-anthesis spike cDNA library
Tissue
Spike before anthesis
Developmental Stage
Adult plant
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE2301-2304_D19_D19ZS Wheat pre-anthesis spike cDNA library Triticum aestivum cDNA clone WHE2301-2304_D19_D19, mRNA sequence.
Other Name
WHE2301-2304_D19_D19ZS  [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli SOLR
DNA Library
TA019E1X
Clone
WHE2301-2304_D19_D19
Probe
BF482744
WHE2301-2304_D19_D19
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Whole spike with awns trimmed, white, green and yellow anther were collected and total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology
TaAffx.37474.1.S1_at184WHE2AFFY
TaAffx.37474.1.S1_at111WHE2AFFY
DNA
agcaaggccaactactagaagacatagcagaagagtactaccatgagcta
atccaccggaatctcctccaaccagatatctcatattttgacatgtctca
ctgcaaaatgcacgacctcttaagacaacttgctttgaatatatcaagag
aagaatgttttgttggagatgttgaatcattaaggggtgaaaatatgcca
aaactgcgacgtgttactgctgttattaagaaggatatgtcagtgttacc
tagagtggataaggtggaagttaaggtgaggactttcctaactgttaagg
gtccatggagaattgagaatacattgttcaagacattcctgcttcttcgt
gttttggtactgaattactcacttgtacaaagcatcccagattacatagg
aaagctgatacatctacgtctacttaatctagattatgctggcatatctt
gtcttccagaatccattggctccctaaagaaccttcaagtactgagcttg
agatggtgtaatgatctgcacagtcttcctttggcaatgaccctgttgtg
cagtttaagatgtcttaatcttttgggcac

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