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GrainGenes Sequence Report: NSFT03P2_Contig13724

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Sequence
BF483693
Contig
NSFT03P2_Contig13724
Tracefile
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External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC295671
wEST map position
BF483693
NCBI UniGene
Ta.54089
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus, strongly similar to NP_568746.1 CLPC (HEAT SHOCK PROTEIN 93-V); ATP binding / ATPase [Arabidopsis thaliana]'
Keyword
EST
Germplasm
Chinese Spring
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
1AL
1DL
2BL
2DL
Clone Library
Wheat pre-anthesis spike cDNA library
Tissue
Spike before anthesis
Developmental Stage
Adult plant
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE2336_G02_N04ZS Wheat pre-anthesis spike cDNA library Triticum aestivum cDNA clone WHE2336_G02_N04, mRNA sequence.
Other Name
WHE2336_G02_N04ZS  [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli SOLR
DNA Library
TA019E1X
Clone
WHE2336_G02_N04
Probe
WHE2336_G02_N04
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Whole spike with awns trimmed, white, green and yellow anther were collected and total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology
TaAffx.110765.1.S1_at504WHE2AFFY
TaAffx.110765.1.S1_at329WHE2AFFY
Ta.1705.1.S1_x_at165WHE2AFFY
Ta.1705.1.S1_x_at56WHE2AFFY
Ta.1705.1.S1_x_at50WHE2AFFY
DNA
acctggtgttgggaaaacagctattgcagaagggcttgcccaacgcattt
ctagtggcgatgtgcctgagacgatagaggggaagaaggtcattaccctt
gatatgggacttcttgttgctggtacgaaataccgtggagaatttgaaga
acggttgaagaaactgatggaagaaatcaaacagagcgatgagataattc
tcttcatcgatgaagttcacaccctcataggagcaggagccgccgaaggt
gctattgatgctgctaatattttaaagccagcattagcaagaggagaact
acagtgtattggagccaccacacttgatgaatacaggaagcatgttgaga
aagacccagcattagaaagacgtttccagccagtaaaggtaccggaacca
tcagttgatgagaccatagaaattctcagagggctccgggagcgatatga
gatccatcataaacttcgttacactgatgattctttgattgcagctgcta
agctatcttatcaatatatcagtgatcgtttcctcccagataaagcaatt
gatttgattgatgaagcaggttcacgtgttaggcttcgacatgcccaggt
tcctgaggaagcc

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