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GrainGenes Sequence Report: BF484617

Sequence
BF484617
Tracefile
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External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
wEST map position
BF484617
NCBI UniGene
Ta.11886
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus, weakly similar to NP_001056018.1 Os05g0511800 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Germplasm
Chinese Spring
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
4AL
7AS
7DS
Clone Library
Wheat pre-anthesis spike cDNA library
Tissue
Spike before anthesis
Developmental Stage
Adult plant
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE2317_F10_K19ZS Wheat pre-anthesis spike cDNA library Triticum aestivum cDNA clone WHE2317_F10_K19, mRNA sequence.
Other Name
WHE2317_F10_K19ZS  [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli SOLR
DNA Library
TA019E1X
Clone
WHE2317_F10_K19
Probe
WHE2317_F10_K19
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Whole spike with awns trimmed, white, green and yellow anther were collected and total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
gtcacctttctcggccccgcgggcgcagaggcacacgccgcgctcgcgtc
gaaggtcgcatccttcgtcctcgccgggcgggggttggcccaggcccacg
gagccaccggcgtctgggtcgacgcctcgctacgcctcagtcctacgttc
gccgacacggccgccgctgtctacaaggccgacgctcgatcggtggactt
cagcaacaagcccagggaggcgatggccgagatcaatgagtggttcgaga
gcaaaacacgcggcctcgtcaagaacattgtctctgagcgcgattgcgac
ggctccacggcgctcgtcctcggcaactcggtcttcttcagcggctactg
gaactcccccttcttcccangggccaccgaggaaggcaggttctatgtca
acgcctcgccggaggatcacactgttcgcgtgccgttcatgaaggggagc
ccgtttcagcagatcggcgtccaccccngcttcaaggtcctgcgcatgcc
ctaccgcggcgactttggcgacagcgacccgttgtttgccatgtacatct
acctcccc

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