GrainGenes Sequence Report: BF484871

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Sequence

BF484871

Tracefile

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External Databases

TIGR Gene Index

NCBI UniGene

DB Remark

Locus Source: Triticum aestivum (bread wheat)

UniGene title 'Transcribed locus, moderately similar to NP_001051174.1 Os03g0733600 [Oryza sativa (japonica cultivar-group)]'

Keyword

EST

Germplasm

Chinese Spring

Species

Triticum aestivum

Cultivar

Chinese Spring

Clone Library

Wheat pre-anthesis spike cDNA library

Tissue

Spike before anthesis

Developmental Stage

Adult plant

Data Source

genbank	Release 135, Apr 15 2003
genbank	Updated Nov 2006

Title

WHE2320_F01_L02ZS Wheat pre-anthesis spike cDNA library Triticum aestivum cDNA clone WHE2320_F01_L02, mRNA sequence.

Other Name

WHE2320_F01_L02ZS [ wEST-mySQL (obsolete) ]

Strain

lab_host E. coli SOLR

DNA Library

TA019E1X

Clone

WHE2320_F01_L02

Probe

[BF484871.1]

{SpCl-639}

Remark

DB_xref: taxon:4565

Feature: source: mol_type = 'mRNA';

Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.

Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Whole spike with awns trimmed, white, green and yellow anther were collected and total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

DNA

tcgccgcagtcgctgatggcggcgcggtcgtcgatgatgtacgcgcagca
ggccatgtcgccgctccagcagcagcagcagcagcagcaccaggcggccg
cgcacggccagctggggatgtcctccggcgcgaccaccgggttcaacctc
ctccacggcgaggccagcatgggcggcggcggcggcggcgccagtggcaa
cagcatgatgaacgccggcgtcttctcggactaccgccgcggcggcagcg
gcgccaaggaggggtcgacctcgctgtcggccgacgctcgcggcgccaac
tctggcgcgcacagcggcgacggggagtacctcaagggcaccgaggagga
aggaagctaggccggcggcatcgtggctagcaccggcatagcagctgcag
gaggaggagtagcaggtagtgttcgagtcgaggatggtagtagcggatta
gcagtacctaaggttgttgcgttggc


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