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GrainGenes Sequence Report: BF485349

Sequence
BF485349
Contig
NSFT03P2_Contig7146
Tracefile
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External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC328468
wEST map position
BF485349
NCBI UniGene
Ta.50519
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus, moderately similar to NP_200810.1 LAC17 (laccase 17); copper ion binding / oxidoreductase [Arabidopsis thaliana]'
Keyword
EST
Germplasm
Chinese Spring
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
1AL
1BL
1DL
Clone Library
Wheat pre-anthesis spike cDNA library
Tissue
Spike before anthesis
Developmental Stage
Adult plant
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE2310_D09_G18ZS Wheat pre-anthesis spike cDNA library Triticum aestivum cDNA clone WHE2310_D09_G18, mRNA sequence.
Other Name
WHE2310_D09_G18ZS  [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli SOLR
DNA Library
TA019E1X
Clone
WHE2310_D09_G18
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Whole spike with awns trimmed, white, green and yellow anther were collected and total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology
Ta.4455.1.A1_at248WHE2AFFY
[ Show all 19 ]
DNA
cggttcgcggcgttcatcaacaacgtctccttcgtgctcccgaccacggc
gctgctgcagtcgcactacaccgggatgtccaagggcgtgtacgcctccg
acttccccttcgcgccgccgcgcccgttcaactacacgggcacgccgccc
aacaacacgaacgtgatgaccgggaccaaggcgctggtgctggggggggg
caccgccgtggagctggtgatgcaggacaccagcatggtgggcgccgaga
gccacccgctgcacctgcacggcttcaacttcttcgtcgtcggccagggc
ttcggcaactacgacccggccaaggaccccgccaagttcaacctcgtcga
ccccgtcgagcgcaacaccgtcggcgtccccgccggcggctgggtcgcca
tccgctttcgcgccgacaacccacgtgtatggctcatgcattgccatttg
g

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