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GrainGenes Sequence Report: BG262560

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Sequence
BG262560
Contig
Ta.3476.1.S1_at
Tracefile
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External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC290051
wEST map position
BG262560
NCBI UniGene
Ta.9910
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus, moderately similar to NP_001049095.1 Os03g0168900 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Germplasm
Chinese Spring
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
1BL
2BS
Clone Library
Wheat 5-15 DAP spike cDNA library
Tissue
Spike
Developmental Stage
Adult plant
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE0937_E06_I11ZS Wheat 5-15 DAP spike cDNA library Triticum aestivum cDNA clone WHE0937_E06_I11, mRNA sequence.
Other Name
WHE0937_E06_I11ZS  [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli SOLR
DNA Library
TA018E1X
Clone
WHE0937_E06_I11
Probe
WHE0937_E06_I11
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Spikes at 5, 10 and 15 DAP were harvested, total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors ).
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Spikes at 5, 10 and 15 DAP were harvested, total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology
Ta.3476.1.S1_at670WHE2AFFY
[ Show all 12 ]
DNA
tggcggcggcgggttggctccggcgagcggcatcggcggtggcgctgcct
cgcatgccgtctggactcaccatcatgccagcccctccccccgctcctct
ccccgaggctcagtccctcgtgctccccggcctcggcgctgccatcgccc
ccgcgatggaactcatggccgtccccaagaagaagatctcaaaatataag
aggggtttgagaaatggacccagagccctgaagcctgttccagtaattgt
ccgttgcaggtgctgcgggcgagtgaagctgccccacttctactgctgca
gcggagaaaaagggaacgccggcgactccagctcataaacaccggctttg
cgcacgaagccgtggcaggacatcttagtgcaatggaaatcaccagatat
cgctttgtattccgctgccttctctttgcgagcagttatctacatagact
ttagtttgatttgcataat

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