GrainGenes Sequence Report: BG262703
Sequence
BG262703
Contig
NSFT03P2_Contig3888
Tracefile
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External Databases
Data at GenBank Data at EMBL Data at DDBJ
wEST map position
BG262703
NCBI UniGene
Ta.48442
DB Remark
Locus Source: Triticum aestivum (bread wheat) UniGene title 'Clone wkm2c.pk005.e4:fis, full insert mRNA sequence'
Keyword
EST
Germplasm
Chinese Spring
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
4BS 4DS
Clone Library
Wheat 5-15 DAP spike cDNA library
Tissue
Spike
Developmental Stage
Adult plant
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
WHE0940_C09_F18ZS Wheat 5-15 DAP spike cDNA library Triticum aestivum cDNA clone WHE0940_C09_F18, mRNA sequence.
Other Name
WHE0940_C09_F18ZS [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli SOLR
DNA Library
TA018E1X
Clone
WHE0940_C09_F18
Probe
WHE0940_C09_F18
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Spikes at 5, 10 and 15 DAP were harvested, total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors ). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Spikes at 5, 10 and 15 DAP were harvested, total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology
Ta.3927.1.S1_at 973 WHE2AFFY
DNA
aagtccacaagctccagtgggccacaatgtggggagcagatactgtcatg
gacctttcaacagggcgccatatccatgagacccgggaatggattattcg
taactcttcggttcctattggaactgttcctatttaccaagcacttgaga
aagttaacggtattgctgaagatctgagctgggaagtctttagggacact
ttaattgaacaagccgagcagggtgttgattacttcaccatccacgctgg
tgtgctgcttcgatatattcctctcacagcaaagagaatgaccggcatag
tttctcgtggtggatcaatccatgcaaaatggtgcttgacgtatcacaag
gagaactttgcttatgagcactgggatgacattcttgacatatgtaacca
gtatgatgtggcattatctattggtgatggcctgaggcctggttccattt
atgatgctaatgatagtgctcagtttgcagaactgctgact
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: BG262703
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||