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GrainGenes Sequence Report: BG263021

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Sequence	BG263021
Contig	TaAffx.94489.1.S1_at
Tracefile	[ Download ] [ View ]
External Databases	Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index	TC277209
wEST map position	BG263021
NCBI UniGene	Ta.2727
DB Remark	Locus Source: Triticum aestivum (bread wheat) UniGene title 'Rab GTP-binding protein'
Keyword	EST
Germplasm	Chinese Spring
Species	Triticum aestivum
Cultivar	Chinese Spring
Chromosome	7DS
Clone Library	Wheat 5-15 DAP spike cDNA library
Tissue	Spike
Developmental Stage	Adult plant
Data Source	genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title	WHE0946_B05_C10ZS Wheat 5-15 DAP spike cDNA library Triticum aestivum cDNA clone WHE0946_B05_C10, mRNA sequence.
Other Name	WHE0946_B05_C10ZS  [ wEST-mySQL (obsolete) ]
Strain	lab_host E. coli SOLR
DNA Library	TA018E1X
Clone	WHE0946_B05_C10
Probe	WHE0946_B05_C10
Remark	DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Spikes at 5, 10 and 15 DAP were harvested, total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors ). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Spikes at 5, 10 and 15 DAP were harvested, total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology	TaAffx.94489.1.S1_at 303 WHE2AFFY TaAffx.90564.1.S1_at 141 WHE2AFFY Ta.2727.1.A1_at 127 WHE2AFFY Ta.8775.1.S1_at 101 WHE2AFFY Ta.20513.1.S1_at 52 WHE2AFFY
DNA	attacaagcgcatattacagaggagctgtcggtgcgcttgttgtgtatga tgtgacacgccatgtgaccttcgagaatgtggagaggtggctgaaggagc tcaaggaccataccgatgctaacattgtgatcatgctagtcggcaacaag gccgacctgcgccacttgctggctgtctctgtggag

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