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GrainGenes Sequence Report: Ta.9381.1.S1_at

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Sequence
BG263044
Contig
Ta.9381.1.S1_at
NSFT03P2_Contig11737
Tracefile
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External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC282671
NCBI UniGene
Ta.9381
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus, moderately similar to NP_001046637.1 Os02g0305700 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Germplasm
Chinese Spring
Species
Triticum aestivum
Cultivar
Chinese Spring
Clone Library
Wheat 5-15 DAP spike cDNA library
Tissue
Spike
Developmental Stage
Adult plant
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE0946_D10_G20ZS Wheat 5-15 DAP spike cDNA library Triticum aestivum cDNA clone WHE0946_D10_G20, mRNA sequence.
Other Name
WHE0946_D10_G20ZS  [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli SOLR
DNA Library
TA018E1X
Clone
WHE0946_D10_G20
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Spikes at 5, 10 and 15 DAP were harvested, total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors ).
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Spikes at 5, 10 and 15 DAP were harvested, total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
ttctgctttaagcgcccaagcaatcctccgtgtcactgtgggtgtccctg
ttgccccaaaagaaggcggcatctctgctgctctgaaagaaggcggcatc
cctgctgccctgaaagaaggtggcatctctgttgtcgttgctccaaaaga
aggtggcgcccctgttgctaccaaagaaagtgctgcggccgtgggctgct
agcgttgggagttggccgacacaagcgatgttgcgattgttccacatcca
attcacgtttttgctgtacatgatttatgggttgctcggtcggtaaatcg
aatctccttttgctggagttcttcaggcttgagggcctggggtgggtgtt
aatattggtgcgagatat

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