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GrainGenes Sequence Report: BG263124

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Sequence
BG263124
Tracefile
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External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
wEST map position
BG263124
NCBI UniGene
Ta.7122
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus'
Keyword
EST
Germplasm
Chinese Spring
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
6AS
Clone Library
Wheat pre-anthesis spike cDNA library
Tissue
Spike before anthesis
Developmental Stage
Adult plant
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE2337_D06_G11ZS Wheat pre-anthesis spike cDNA library Triticum aestivum cDNA clone WHE2337_D06_G11, mRNA sequence.
Other Name
WHE2337_D06_G11ZS  [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli SOLR
DNA Library
TA019E1X
Clone
WHE2337_D06_G11
Probe
MAG2282
WHE2337_D06_G11
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Whole spike with awns trimmed, white, green and yellow anther were collected and total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology
Ta.7122.3.S1_at244WHE2AFFY
Ta.7122.3.S1_at180WHE2AFFY
DNA
aactcttcatctttgtctagcagcatcagaattacagtctagcagtatct
cggtaagaaacatattgcatgataaataactaatgccactggggagacag
tatgtgaagattggattattgcaagaagacaaaaagctttggaaatcaag
tgccatgctctgttttttttgcaggtcacaaaatgctttggatagcatat
gtacttctgtcagttgcattccgtatcttttatctcatctattttatttt
gattaggagattgaaaaaataagggtaaccgttctgcgttggaaataatt
tctagttaaaaaagcatgccatggcttaacctgataaacacatgataagt
ctccttttgtgattcagggaaaggaaaacagcatcatcccacgttcttac
attcagatttctgagcagagagatagtagtttgtaaggtctcggtcaaac
tgcagagctctgttaatgatgggggagcacttgttgtc

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