GrainGenes Sequence Report: BG274844
Sequence
BG274844
Contig
NSFT03P2_Contig7240
Tracefile
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External Databases
Data at GenBank Data at EMBL Data at DDBJ
wEST map position
BG274844
DB Remark
Locus Source: Aegilops speltoides
Keyword
EST
Species
Aegilops speltoides
Cultivar
F2 from 2-12-4-8-1-1-1-(1) x PI36909-12-811-(1 ) F2 from 2-12-4-8-1-1-1-(1) x PI36909-12-811-(1)
Chromosome
2BS 2DS
Clone Library
Aegilops speltoides anther cDNA library
Tissue
Anther
Developmental Stage
Premeiotic anthers
Data Source
genbank Release 135, Apr 15 2003 genbank Downloaded 2008-2009
Title
WHE2234_H11_O22ZS Aegilops speltoides anther cDNA library Aegilops speltoides cDNA clone WHE2234_H11_O22, mRNA sequence.
Other Name
WHE2234_H11_O22ZS [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli SOLR
DNA Library
AS040E1X
Clone
WHE2234_H11_O22
Probe
WHE2234_H11_O22
Remark
DB_xref: taxon:4573 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in a growth chamber at the University of California, Davis (Akhunov). Premeiotic anthers were harvested, total RNA and poly(A) RNA were prepared, from each tissue and then pooled, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Akhunov, Chin, Choi, Close, Fenton, Kianian, Otto, Simons , Zhang) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in a growth chamber at the University of California, Davis (Akhunov). Premeiotic anthers were harvested, total RNA and poly(A) RNA were prepared, from each tissue and then pooled, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Akhunov, Chin, Choi, Close, Fenton, Kianian, Otto, Simons, Zhang) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology
Ta.18635.1.S1_at 111 WHE2AFFY
[ Show all 9 ]
DNA
gtttaatatctgatatgtggggtacacccccacaacgatattaaatttat
tttttatgggggagggtccaccacagtggcttgccactgggccccttagg
tgtcgcccgtgtcttgcactaccgcacgggctggcgcaccccaaccaaac
caattcaatttttctatactggtgggaccggccagtcagcttgggtaaac
gatcgtttggttctgtgtatggactcacactgcagcgttggtctctcgaa
cggctggtcctgatccagagcgatgcgatgcagtctcgctggccaggttg
taaaccgaggagcgaacgacacagccatacatgcatgggtacgcagtaag
cataccgttgattaatttgcaaccacgacgcggaatgcaacgtccattgc
tgtttctgcttttgatttgacagtttgaagagcaagcaaacaagaagcac
aagaaggtttatgggttgatccctttgttggtcgatccgttnctataaac
ggaatttcaaaataaaaagtggcaacatgtgaacccaaacttgac
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: BG274844
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||