GrainGenes Sequence Report: BG301298

HVSMEb0020I24f Hordeum vulgare seedling shoot EST library HVcDNA0002 (Dehydration stress) Hordeum vulgare subsp. vulgare cDNA clone HVSMEb0020I24f, mRNA sequence.

Strain

lab_host TJC121

vulgare

Clone

HVSMEb0020I24f

Probe

BG301298

Remark

DB_xref: taxon:112509

Feature: source: mol_type = 'mRNA';

Locus Comment: Contact: Wing RA; Clemson University Genomics Institute; Clemson University; 100 Jordan Hall, Clemson, SC 29634, USA; Tel: 864 656 7288; Fax: 864 656 4293; Email: rwing@clemson.edu; Total hq bases = 308; Seq primer: AATTAACCCTCACTAAAGGG; High quality sequence stop: 704.

Note: Vector: lambdaZAP; Site_1: EcoR1; Site_2: Xho1; Seeds were surface sterilized then germinated under axenic conditions in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Five-day old seedlings were incubated at 90% RH for 24 hr. Shoots were then harvested, total RNA was prepared, poly(A) RNA was purified, one primary unamplified cDNA library was made, 600000 pfu were in vivo excised to give pBluescript SK(-) cDNA phagemids. These steps were performed in the TJ Close laboratory at the University of California, Riverside (Choi, Close, Fenton). Phagemids were plated and picked at the Clemson University Genomics Institute (CUGI) (Begum, Palmer, Frisch, Atkins and Wing). Plasmid DNA preparations, DNA sequencing and sequence analysis were performed at CUGI (Wing, Yu, Frisch, Henry, Simmons, Oates, Rambo, Main). The sequence has been trimmed to remove vector sequence and contains a minimum of 100 bases of phred value 20 or above. For more details on library preparation and sequence analysis see http:

DNA

ctccctcctcctctctctctctctctgctaaggaaggaagaaagaaagcc
acaagcacgcaagcagaggaagaagaagaacggaaccatgtcttccccaa
gcaagcggagggagatggatctcatgaagcttatgatgagtgattacaag
gtggacatgatcaacgactggatgcaggagttcttcgttcacttccaccg
accaaacgacagtatttaccactgtggtgtgttgaacgtgagggttgagc
tgactgaagcttacccttacaaatccccttccattggcttcaccaacaag
atctatcaccccaatgtcgatgagatgtccggatcggtctgcttggacgt
gatcaaccagacctggagcccaatgtttgacctagtgaatatctttgaag
tgttcctgccccagcttctactctaccctaacccatcggacccattgaat
ggcgaggcggcatcgctgatgatgcgcgacaagaatgcctatgaacaaaa
agtcaaagaatattgtgagagatatgccaaggctgaagacatatccccag
aggaggaagaggaggagagcgacaaggagccgagcgacgccgaggtctgc
gactccggcgacgaggccatcatgggccacgcagacccttgactggtggt
tggatggttagctcaatcagtaactcagtattgcaagatgatcatgatgt
atctctgtctgtacatagctgcggcgctaactcatgccgtcatggcagat
tctgaagaaaggtatcagccatctcaagtcaatttcatctccctagtccc
cccctgtcgccgaatcggaatcgtctgtggaatacttgccttttgttggg
gttggttccatttgcactgccattcatggtttcatggtaggagaagttgg
ctct


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