GrainGenes Sequence Report: BG310452

HVSMEc0018P18f Hordeum vulgare seedling shoot EST library HVcDNA0003 (Etiolated and unstressed) Hordeum vulgare subsp. vulgare cDNA clone HVSMEc0018P18f, mRNA sequence.

Strain

lab_host TJC121

vulgare

Clone

HVSMEc0018P18f

Probe

BG310452

Remark

DB_xref: taxon:112509

Feature: source: mol_type = 'mRNA';

Locus Comment: On Feb 22, 2001 this sequence version replaced gi:13111299.; Contact: Wing RA; Clemson University Genomics Institute; Clemson University; 100 Jordan Hall, Clemson, SC 29634, USA; Tel: 864 656 7288; Fax: 864 656 4293; Email: rwing@clemson.edu; Total hq bases = 320; Seq primer: AATTAACCCTCACTAAAGGG; High quality sequence stop: 404.

Note: Vector: lambdaZAP; Site_1: EcoR1; Site_2: Xho1; Seeds were surface sterilized then germinated under axenic conditions in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Five-day old seedling shoots were then harvested, total RNA was prepared, poly(A) RNA was purified, one primary unamplified cDNA library was made, and 1 million pfu were in vivo excised to give pBluescript SK(-) cDNA phagemids. These steps were performed in the TJ Close laboratory at the University of California, Riverside (Choi, Close, Fenton). Phagemids were plated and picked at the Clemson University Genomics Institute (CUGI) (Begum, Palmer, Frisch, Atkins and Wing). Plasmid DNA preparations, DNA sequencing and sequence analysis were performed at CUGI (Wing, Yu, Frisch, Henry, Simmons, Oates , Rambo, Main). The sequence has been trimmed to remove vector sequence and contains a minimum of 100 bases of phred value 20 or above. For more details on library preparation and sequence analysis see http:

Note: Vector: lambdaZAP; Site_1: EcoR1; Site_2: Xho1; Seeds were surface sterilized then germinated under axenic conditions in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Five-day old seedling shoots were then harvested, total RNA was prepared, poly(A) RNA was purified, one primary unamplified cDNA library was made, and 1 million pfu were in vivo excised to give pBluescript SK(-) cDNA phagemids. These steps were performed in the TJ Close laboratory at the University of California, Riverside (Choi, Close, Fenton). Phagemids were plated and picked at the Clemson University Genomics Institute (CUGI) (Begum, Palmer, Frisch, Atkins and Wing). Plasmid DNA preparations, DNA sequencing and sequence analysis were performed at CUGI (Wing, Yu, Frisch, Henry, Simmons, Oates, Rambo, Main). The sequence has been trimmed to remove vector sequence and contains a minimum of 100 bases of phred value 20 or above. For more details on library preparation and sequence analysis see http:

DNA

tgccggtcggtgctccagcatttcaatcgccaacaccggcaaggaaagca
agtcaccatgggcaacaacaaggccgcggcctgcgtgctgctgctcgtcc
tgtgcggcctgctcgccgcggacacggcggcggcggcggggtgcgacgcg
ggcgggctgagcccgtgcgtgggcgccatcatgctgggcggggcggtgac
tccagggtgctgcgcccggctgcgcgcccagcgggcgtgcctgtgccagt
acgcgcgcgacccggagtaccgcggctacgtcaacagccccagggcgcat
agcgtcgtcaccgcgtgcgggctcccaaggcccaagtgctgattatacgt
cccgtgaggccggagttgtgcgcgatgccaaacaccagtggacggaagtc
tatatacgtaatgtaacagtgtggttaatcatacggtccacgacaccatg
cagctggaacgtacatacgtgtccgcgcgtccgtgtatgggtgaatgggt
gacatctaaataaacggggctcgctctaacctgccacccgtataaacgtg
ccccggagtcaaggggccttctccctaccgtatccggcaaagggataaac
agaagcagaaacctccccccgcacaaaaagggcggggcccaggcaacgcg
cactcgcgaactcactgcgggtacaggggacccgccacaggaaaaaagcc
gcgcaaccggggtcaaccgacccggaacaagagcgatgaccctcgagaag
agcaacgtcccccatgctgtactagaggaaaccgcgccccggactctcga
ccgcacaagcacgaagacgcgggagcgaacaag


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