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GrainGenes Sequence Report: BG313162

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Sequence
BG313162
Contig
Ta.10498.1.S1_a_at
Ta.10498.1.S1_at
NSFT03P2_Contig15376
Tracefile
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External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC305629
wEST map position
BG313162
NCBI UniGene
Ta.10498
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus, strongly similar to NP_001049645.1 Os03g0265100 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Germplasm
Chinese Spring
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
4AS
4BL
4DL
Clone Library
Wheat salt-stressed sheath cDNA library
Tissue
Sheath
Developmental Stage
Adult plant
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE2054_E05_J10ZS Wheat salt-stressed sheath cDNA library Triticum aestivum cDNA clone WHE2054_E05_J10, mRNA sequence.
Other Name
WHE2054_E05_J10ZS  [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli SOLR
DNA Library
TA037E1X
Clone
WHE2054_E05_J10
Probe
WHE2054_E05_J10
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown under hydroponic conditions at UC Davis, salt stressed for 12 hours, and for 7 days, then dissected and frozen (Akhunov in J Dvorak Lab). Total RNA was prepared from sheath tissue, equal quantities of RNA were pooled from the two samples, polyA was purified from the pooled RNA, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab at the University of California, Riverside (Akhunov, Chin , Choi, Close, Fenton, Kianian, Otto, Simons, Zhang). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown under hydroponic conditions at UC Davis, salt stressed for 12 hours, and for 7 days, then dissected and frozen (Akhunov in J Dvorak Lab). Total RNA was prepared from sheath tissue, equal quantities of RNA were pooled from the two samples, polyA was purified from the pooled RNA, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab at the University of California, Riverside (Akhunov, Chin, Choi, Close, Fenton, Kianian, Otto, Simons, Zhang). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology
Ta.10498.1.S1_a_at1037WHE2AFFY
Ta.10498.1.S1_at1037WHE2AFFY
Ta.10498.1.S1_at113WHE2AFFY
TaAffx.8667.1.S1_at182WHE2AFFY
TaAffx.8667.1.S1_at100WHE2AFFY
DNA
tatggtttttggcgcccttgctatcgcaaagagatttcagttccaatggt
catgtcttatcacacacatcttccagcgtacttaccagggtacaatttaa
attggttacttggacccacatggggccttataagatgtctccacaggtct
gcagatcttactctagttccttcagtagctattgccgaggactttgaaac
tgctaaagtagtaccagcaaacagagtacggctttggaacaagggtgttg
attctgaaagcttccatcctaaatttcggaggcatgaaatgcggatcaag
ttgagcggtggtgaaccagaaaaaccattgataattcacgtgggccgttt
tgggcgtgaaaagaatttggattttctgaagagagttatggaaaagctcc
ctggagtaagaattgcttttgttggagatggaccatacagggctgagctg
gaaaaaatgttcacaggcatgcctgcagttttcaccggaatgctccaagg
cgaggagctctctcaagcgtacgccagtggggacgtatttgcaatgcctt
cagagtctgagaccctcggacaagtagtgctggagtccatggcttctgga
g

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