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GrainGenes Sequence Report: BG313298

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Sequence	BG313298
Contig	Ta.21562.1.S1_at NSFT03P2_Contig5205
Tracefile	[ Download ] [ View ]
External Databases	Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index	TC318500
wEST map position	BG313298
DB Remark	Locus Source: Triticum aestivum (bread wheat)
Keyword	EST
Germplasm	Chinese Spring
Species	Triticum aestivum
Cultivar	Chinese Spring
Chromosome	6AL 6DL
Clone Library	Wheat salt-stressed sheath cDNA library
Tissue	Sheath
Developmental Stage	Adult plant
Data Source	genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title	WHE2053_C02_F03ZS Wheat salt-stressed sheath cDNA library Triticum aestivum cDNA clone WHE2053_C02_F03, mRNA sequence.
Other Name	WHE2053_C02_F03ZS  [ wEST-mySQL (obsolete) ]
Strain	lab_host E. coli SOLR
DNA Library	TA037E1X
Clone	WHE2053_C02_F03
Probe	WHE2053_C02_F03
Remark	DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown under hydroponic conditions at UC Davis, salt stressed for 12 hours, and for 7 days, then dissected and frozen (Akhunov in J Dvorak Lab). Total RNA was prepared from sheath tissue, equal quantities of RNA were pooled from the two samples, polyA was purified from the pooled RNA, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab at the University of California, Riverside (Akhunov, Chin , Choi, Close, Fenton, Kianian, Otto, Simons, Zhang). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown under hydroponic conditions at UC Davis, salt stressed for 12 hours, and for 7 days, then dissected and frozen (Akhunov in J Dvorak Lab). Total RNA was prepared from sheath tissue, equal quantities of RNA were pooled from the two samples, polyA was purified from the pooled RNA, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab at the University of California, Riverside (Akhunov, Chin, Choi, Close, Fenton, Kianian, Otto, Simons, Zhang). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology	Ta.21562.1.S1_at 646 WHE2AFFY Ta.21562.1.S1_at 513 WHE2AFFY
DNA	atttgtgggccatggggtaggcaaagtgtttcatgcggagcctgcagtgc ttcatttccgcaataatgaaaagggccgcatgattttgaaccaaacattt accatagagccgatgctaaccatagggagcacaaactcgaccatatggtc cgacgactggactgcggtgacggaggacgggagcctgtgagggcggtgtg agcacacgctactgatcactgaagacggcgtggaaatgggggggcagtgt taagcccggccggagaacaaagaggtcaatgagtgaaaggctggatggca gggacggcagaacctgcattttttttctgggactacacatggtccaatca aactccaagtgcaaaactacacttttttttcttgatacatcagtcggttg atgcatagattatatacaaagttttgcatcgttgttgggat

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