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GrainGenes Sequence Report: BG313725

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Sequence	BG313725
Tracefile	[ Download ] [ View ]
External Databases	Data at GenBank Data at EMBL Data at DDBJ
wEST map position	BG313725
NCBI UniGene	Ta.51090
DB Remark	Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus'
Keyword	EST
Germplasm	Chinese Spring
Species	Triticum aestivum
Cultivar	Chinese Spring
Chromosome	1BL 2BS 6BL
Clone Library	Wheat salt-stressed sheath cDNA library
Tissue	Sheath
Developmental Stage	Adult plant
Data Source	genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title	WHE2057_G03_N05ZS Wheat salt-stressed sheath cDNA library Triticum aestivum cDNA clone WHE2057_G03_N05, mRNA sequence.
Other Name	WHE2057_G03_N05ZS  [ wEST-mySQL (obsolete) ]
Strain	lab_host E. coli SOLR
DNA Library	TA037E1X
Clone	WHE2057_G03_N05
Probe	WHE2057_G03_N05
Remark	DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown under hydroponic conditions at UC Davis, salt stressed for 12 hours, and for 7 days, then dissected and frozen (Akhunov in J Dvorak Lab). Total RNA was prepared from sheath tissue, equal quantities of RNA were pooled from the two samples, polyA was purified from the pooled RNA, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab at the University of California, Riverside (Akhunov, Chin , Choi, Close, Fenton, Kianian, Otto, Simons, Zhang). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown under hydroponic conditions at UC Davis, salt stressed for 12 hours, and for 7 days, then dissected and frozen (Akhunov in J Dvorak Lab). Total RNA was prepared from sheath tissue, equal quantities of RNA were pooled from the two samples, polyA was purified from the pooled RNA, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab at the University of California, Riverside (Akhunov, Chin, Choi, Close, Fenton, Kianian, Otto, Simons, Zhang). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology	Ta.484.1.S1_a_at 222 WHE2AFFY Ta.484.1.S1_at 222 WHE2AFFY TaAffx.32232.1.S1_at 153 WHE2AFFY TaAffx.12962.1.S1_at 80 WHE2AFFY
DNA	atgattttgatgacaatttgttagtgtatttctagctggaattgcattat atcttacaaattcacaatgcaaccaattaataaggagaatagaagcattt gcatccatctaacatatttgtatgatattttttcaaaagctcaaggttgt agatgtggcgctgtatgaggcgatcattaggatggaaaataaattaatgg gagaatattgtttgaatttccttcgttctgtaaatggcaatacaagctat gtttgcgccatgattctattccaatttttttaataactggtcattaacga cagataaaatgttctgtattgaaatggagcatttgtcagtttgggtttag taggtcagaatggttctattctacagcacgattatgtaaccagttcaaga attgatgagtttttgagccctatg

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