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GrainGenes Sequence Report: BG314071

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Sequence	BG314071
Contig	NSFT03P2_Contig2774
Tracefile	[ Download ] [ View ]
External Databases	Data at GenBank Data at EMBL Data at DDBJ
wEST map position	BG314071
DB Remark	Locus Source: Triticum monococcum
Keyword	EST
Germplasm	T. monococcum ssp. monococcum DV92
Species	Triticum monococcum
Cultivar	DV92
Chromosome	4AS
Clone Library	Triticum monococcum early reproductive apex cDNA library
Tissue	Early reproductive apex
Developmental Stage	Seven week-old plants
Data Source	genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title	WHE2467_G05_M09ZS Triticum monococcum early reproductive apex cDNA library Triticum monococcum cDNA clone WHE2467_G05_M09, mRNA sequence.
Other Name	WHE2467_G05_M09ZS  [ wEST-mySQL (obsolete) ]
Strain	lab_host E. coli XLOLR
DNA Library	TM043E1X
Clone	WHE2467_G05_M09
Remark	DB_xref: taxon:4568 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; The tissue, total RNA, and poly(A) RNA were prepared from apex at double-ridge stage to terminal-spikelet stage during transition from vegetative state to flower state, a cDNA library was made, and the cDNA clones were in vivo excised at the University of California, Davis (V. Echenique, B. Stamova , J. Dubcovsky). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; The tissue, total RNA, and poly(A) RNA were prepared from apex at double-ridge stage to terminal-spikelet stage during transition from vegetative state to flower state, a cDNA library was made, and the cDNA clones were in vivo excised at the University of California, Davis (V. Echenique, B. Stamova, J. Dubcovsky). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology	Ta.26697.1.A1_at 345 WHE2AFFY Ta.26697.1.A1_at 214 WHE2AFFY Ta.25749.1.A1_at 48 WHE2AFFY
DNA	gctgaactgtaaatatccatacaactagtctcaaaataaaacggtatcca ggaataagactccggtaggtagaaatacctagtgcaacagtgtctaggag cagagcacaaatatttacagttccgccaaaacttgtggtagggtatgatg gaccatcaagctcggcgcttgctgagacgccatgagttgggctcgtggta cctgtcgtacagaggctcaatcctttcctgtcgcttcctcttgctcatct tggttggatctgcgcccttgtagaatcttggcgctaactccactaaccat ttcgggtcaaccacggtcacctccctcatatactccttggtcgtcattac aagctcgtggtaaatgacccaatctggttggcgctggaacagcgcactgc ttgggtgaatgtacactggctggttctccaccaaggttctgtagccttcc tggggatctttcctggcagcatggaaaaagaagccggcagtgattgcttt ccttattttcatgaagttcttcccagcagcgacaacatccaacttatatc tgtccatgatagttagaagctgctntctgacatcttgtgctctccttagt gatcttgactgaac

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