GrainGenes Sequence Report: BG367514

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Sequence

BG367514

External Databases

NCBI UniGene

DB Remark

Locus Source: Hordeum vulgare subsp. vulgare

UniGene title 'CDNA clone: FLbaf177o22, mRNA sequence'

Keyword

EST

Species

Hordeum vulgare subsp. vulgare

Cultivar

Morex

Clone Library

Hordeum vulgare 20 DAP spike EST library HVcDNA0010 (20 DAP)

Tissue

20 DAP spike

Data Source

genbank	Release 135, Apr 15 2003
genbank	Updated Nov 2006

Title

HVSMEi0012I13f Hordeum vulgare 20 DAP spike EST library HVcDNA0010 (20 DAP) Hordeum vulgare subsp. vulgare cDNA clone HVSMEi0012I13f, mRNA sequence.

Strain

lab_host SOLR

vulgare

Clone

HVSMEi0012I13f

Probe

BG367514

Remark

DB_xref: taxon:112509

Feature: source: mol_type = 'mRNA';

Locus Comment: On Mar 8, 2001 this sequence version replaced gi:13256613.; Contact: Wing RA; Clemson University Genomics Institute; Clemson University; 100 Jordan Hall, Clemson, SC 29634, USA; Tel: 864 656 7288; Fax: 864 656 4293; Email: rwing@clemson.edu; Total hq bases = 507; Seq primer: AATTAACCCTCACTAAAGGG; High quality sequence start: 6; High quality sequence stop: 651.

Note: Vector: lambdaZAP; Site_1: EcoR1; Site_2: Xho1; Plants were grown in the greenhouse at the University of California, Riverside (Fenton, SJ Close, TJ Close). Whole spikes with awns trimmed were collected at 20 DAP (Fenton ). Total RNA was prepared, poly(A) RNA was purified, one primary unamplified cDNA library was made, and 1 million pfu were in vivo excised to give pBluescript SK(-) cDNA phagemids in the TJ Close lab at the University of California, Riverside (Choi). Phagemids were plated and picked at the Clemson University Genomics Institute (CUGI) (Begum, Palmer, Frisch, Atkins and Wing). Plasmid DNA preparations, DNA sequencing and sequence analysis were performed at CUGI (Wing, Yu, Frisch, Henry, Simmons, Oates , Rambo, Main). The sequence has been trimmed to remove vector sequence and contains a minimum of 100 bases of phred value 20 or above. For more details on library preparation and sequence analysis see http:

Note: Vector: lambdaZAP; Site_1: EcoR1; Site_2: Xho1; Plants were grown in the greenhouse at the University of California, Riverside (Fenton, SJ Close, TJ Close). Whole spikes with awns trimmed were collected at 20 DAP (Fenton). Total RNA was prepared, poly(A) RNA was purified, one primary unamplified cDNA library was made, and 1 million pfu were in vivo excised to give pBluescript SK(-) cDNA phagemids in the TJ Close lab at the University of California, Riverside (Choi). Phagemids were plated and picked at the Clemson University Genomics Institute (CUGI) (Begum, Palmer, Frisch, Atkins and Wing). Plasmid DNA preparations, DNA sequencing and sequence analysis were performed at CUGI (Wing, Yu, Frisch, Henry, Simmons, Oates, Rambo, Main). The sequence has been trimmed to remove vector sequence and contains a minimum of 100 bases of phred value 20 or above. For more details on library preparation and sequence analysis see http:

DNA

agcaaccatcgaagtatgtcaaaatttgggagggaactttgtctgggcaa
aggcaaggacaacctgtatttatttgtaaacttgaaggttataggagtgg
aacatcatctgacgcgcttgcttcagactggccataaacaatgcagatcg
tgcgcctcatagcccaagagcatatgaataacaagcagtatgttggtaag
gcagactttctagtatttcggacattgaatcagcatgggttcctcggaca
actacaagagaagaaactgtgtgcagtgattcagctaccttcacaaactt
tgctgttgtcagtgtctgacaaagctgggcgcctcataggaatgctgttc
ccaggggatatggtggtattcaaaccacaggtctcgactcagcagccaca
gatgcagccacagcagcagcagcagctgcaacagcagcagcaccaaatac
agcagcaacagcagcaactacagcaactgcaacagcagcaactacagcag
caccaaatgcaaatgcagcctcaaggccagcaacttcagcagcagcagca
gatgcagcaaatgcaaccagcaacaacaacagatgcagcaaatgcaacac
caacaacagcagcagcagattcaacagcagcagcagatgcagcagcagca
gcaacaacaacagcaacagatgcagcagatgcagcaacagcagcagcaac
agcctcaat


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