GrainGenes Sequence Report: BG368958

Locus Comment: Contact: Wing RA; Clemson University Genomics Institute; Clemson University; 100 Jordan Hall, Clemson, SC 29634, USA; Tel: 864 656 7288; Fax: 864 656 4293; Email: rwing@clemson.edu; Total hq bases = 510; Seq primer: AATTAACCCTCACTAAAGGG; High quality sequence start: 2; High quality sequence stop: 620.

Note: Vector: lambdaZAP; Site_1: EcoR1; Site_2: Xho1; Plants were grown in the greenhouse at the University of California, Riverside (Fenton, SJ Close, TJ Close). Whole spikes with awns trimmed were collected at 20 DAP (Fenton ). Total RNA was prepared, poly(A) RNA was purified, one primary unamplified cDNA library was made, and 1 million pfu were in vivo excised to give pBluescript SK(-) cDNA phagemids in the TJ Close lab at the University of California, Riverside (Choi). Phagemids were plated and picked at the Clemson University Genomics Institute (CUGI) (Begum, Palmer, Frisch, Atkins and Wing). Plasmid DNA preparations, DNA sequencing and sequence analysis were performed at CUGI (Wing, Yu, Frisch, Henry, Simmons, Oates , Rambo, Main). The sequence has been trimmed to remove vector sequence and contains a minimum of 100 bases of phred value 20 or above. For more details on library preparation and sequence analysis see http:

Note: Vector: lambdaZAP; Site_1: EcoR1; Site_2: Xho1; Plants were grown in the greenhouse at the University of California, Riverside (Fenton, SJ Close, TJ Close). Whole spikes with awns trimmed were collected at 20 DAP (Fenton). Total RNA was prepared, poly(A) RNA was purified, one primary unamplified cDNA library was made, and 1 million pfu were in vivo excised to give pBluescript SK(-) cDNA phagemids in the TJ Close lab at the University of California, Riverside (Choi). Phagemids were plated and picked at the Clemson University Genomics Institute (CUGI) (Begum, Palmer, Frisch, Atkins and Wing). Plasmid DNA preparations, DNA sequencing and sequence analysis were performed at CUGI (Wing, Yu, Frisch, Henry, Simmons, Oates, Rambo, Main). The sequence has been trimmed to remove vector sequence and contains a minimum of 100 bases of phred value 20 or above. For more details on library preparation and sequence analysis see http:

DNA

gcgccccccgcacccgctgcgactggactggactggactggagtggagcg
cactccagtggagacagctgcggaccaggccctccgatccgcccgcgccc
acgcccgccgcagccccctatggtcccgccgcccgccggaccgcctcgcc
gccgcaccaacccggtacgtactgtaataacatgttcagtgctctgaaag
tttcagagtcataattccaagtcagctcaatgacctcatttggtgtcgca
ccggcatctgggctgagagatgctggtggtagtagtgaagtagatagatt
gccggatgaaattagtaatatgaggataagtgatgaaaaggaagtagagg
caacaatcatcaatgggaatggaacagaagctggtcatattatagtcaca
actatcggaggcagagatggccaaaggaagcagacaataagttacatggc
tgaacgtgtcattggtcaaggatcatttggtgttgtgttccaggcaaaat
gtttggagacaagtgagacagtagctatcaagaaggtccttcaggataag
agatacaagaaccgcgagttgcaaatgatgcgtctacttgaccatcccaa
tgtcgtctctctgaaacactgcttcttctcaaccactgaaaaggatgaac
tctttctgaatcttgtgcttgagtatgttcctgagacagtcccccggtca
tctgtaccttcaacaagatgaaccaacgcttgcccctggtttatgtggaa
actatatttttatcagaattggaaggcactggtcttatttcctacccaac
tgtggggttggccccgggacaaaaaccac


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