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GrainGenes Sequence Report: BG418412

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Sequence	BG418412
External Databases	Data at GenBank Data at EMBL Data at DDBJ
NCBI UniGene	Hv.5041
DB Remark	Locus Source: Hordeum vulgare subsp. vulgare UniGene title 'CDNA clone: FLbaf18a08, mRNA sequence'
Keyword	EST
Species	Hordeum vulgare subsp. vulgare
Cultivar	Morex
Clone Library	Hordeum vulgare testa/pericarp EST library HVcDNA0013 (normal)
Tissue	testa/pericarp
Data Source	genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title	HVSMEk0022J18f Hordeum vulgare testa/pericarp EST library HVcDNA0013 (normal) Hordeum vulgare subsp. vulgare cDNA clone HVSMEk0022J18f, mRNA sequence.
Strain	lab_host TJC121 vulgare
Clone	HVSMEk0022J18f
Probe	[Bcl88ct356cn1572] {SpCl-181}
Remark	DB_xref: taxon:112509 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Wing RA; Clemson University Genomics Institute; Clemson University; 100 Jordan Hall, Clemson, SC 29634, USA; Tel: 864 656 7288; Fax: 864 656 4293; Email: rwing@clemson.edu; Total hq bases = 405; Seq primer: AATTAACCCTCACTAAAGGG; High quality sequence stop: 627. Note: Vector: lambdaZAP; Site_1: EcoR1; Site_2: Xho1; Plants were raised from seeds in a Controlled Environments growth chamber maintained in continuous light at 18oC, and testa and pericarp were dissected from developing kernels at Washington State University, Pullman, WA (Kannangara, von Wetstein). Total RNA was prepared, poly(A) RNA was purified, one cDNA library was made, and 1 million pfu were in vivo excised to give pBluescript SK(-) cDNA phagemids in the TJ Close lab at the University of California, Riverside (Akhunov, Chin, Choi, Close, Fenton, Kianian, Otto, Simons, Zhang). Phagemids were plated and picked at the Clemson University Genomics Institute (CUGI) (Begum, Palmer, Frisch, Atkins and Wing). Plasmid DNA preparations, DNA sequencing and sequence analysis were performed at CUGI (Wing, Yu, Frisch, Henry, Simmons, Oates , Rambo, Main). The sequence has been trimmed to remove vector sequence and contains a minimum of 100 bases of phred value 20 or above. For more details on library preparation and sequence analysis see http: Note: Vector: lambdaZAP; Site_1: EcoR1; Site_2: Xho1; Plants were raised from seeds in a Controlled Environments growth chamber maintained in continuous light at 18oC, and testa and pericarp were dissected from developing kernels at Washington State University, Pullman, WA (Kannangara, von Wetstein). Total RNA was prepared, poly(A) RNA was purified, one cDNA library was made, and 1 million pfu were in vivo excised to give pBluescript SK(-) cDNA phagemids in the TJ Close lab at the University of California, Riverside (Akhunov, Chin, Choi, Close, Fenton, Kianian, Otto, Simons, Zhang). Phagemids were plated and picked at the Clemson University Genomics Institute (CUGI) (Begum, Palmer, Frisch, Atkins and Wing). Plasmid DNA preparations, DNA sequencing and sequence analysis were performed at CUGI (Wing, Yu, Frisch, Henry, Simmons, Oates, Rambo, Main). The sequence has been trimmed to remove vector sequence and contains a minimum of 100 bases of phred value 20 or above. For more details on library preparation and sequence analysis see http:
DNA	gagagagagagagagagagagagagagagagagagagagagagagagaga gaggtttggggaaactaagacgccttgcccttatatgtgctgttgaacta atagaaacatacggttagcaaaggaaagattgccaaagcatgtatgtcag caaccatcaatcagaaagcgaagaagagtatccagtcggagaaattgtct gttgcagttagcgaaagaacacaggggctgctctttctgattttcatggc ttaatctgctgtgagcctaatgatggccagtttgctttgctggaggatgc agtgttattgaagtatttttgttgtttgtatgtatgtggagggtaaataa tagtacctgttatcttgaaaagaacggagcaaaatgtattgtgttcccta tttgttagtttgattttcaactgaaggtggccttgaagatataatgaagt gcaaaannnnnaatnantnnntatattaaaaaaaaataaattattaatat nattttttattttttnttattttttgttttttttcgatatttgtttgnnt ttttntggggggtggtgggggtttttctcctctttctttttgtgtggagt ttttcccctcatcctcgcctttcttttttctaaaactttgagccgaattt aaattgggtgcgtttcgaaaattataaaatgtaggggaaatataacaatc ctgttctatgatcgaagtgtgattcattgtgatataaggtacttgtgagt gattgttttaataaattaggtgaatccactttattatggactttatttct tagcatatcccatttatatgtcgttgggccgcacgtcgtttgttcttgtg tttcaactagatgtggcttatcggccgttagggtggtgacggaaggttgt ctttttgtataagaagatttagacgacccg

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