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GrainGenes Sequence Report: NSFT03P2_Contig13986

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Sequence
BG605112
Contig
Ta.9286.1.S1_at
NSFT03P2_Contig13986
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC334055
NCBI UniGene
Ta.9286
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus, moderately similar to NP_001046077.1 Os02g0178500 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Triticum aestivum
Cultivar
Chinese Spring
Clone Library
Wheat pre-anthesis spike cDNA library
Tissue
Spike before anthesis
Developmental Stage
Adult plant
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE2327_E02_J03ZS Wheat pre-anthesis spike cDNA library Triticum aestivum cDNA clone WHE2327_E02_J03, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE2327_E02_J03
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Whole spike with awns trimmed, white, green and yellow anther were collected and total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology
Ta.9286.1.S1_at795WHE2AFFY
Ta.9286.1.S1_at90WHE2AFFY
Ta.4636.1.S1_a_at50WHE2AFFY
DNA
ggcatggaattgtgcagaacttggctagttcgatggagtccccagtgttg
gaatggacccagggccatcagttctccaggggaacaaacttccagtgctt
tgcatcaactggcgatgggtctattgtggtaggctcattggacgggaaaa
tccgtttgtactcaaagagctctatgaggatggcgaagacggccttccca
gggctgggttcaccaattactcatgtggatgtgacgtacgatgggaagtg
gatactgggaaccactgatacctatctgatactcatatgcaccattttca
ttgacaaggatgggaaggagaagacagggtttggtggaagaatggggaac
cgaattgctgcaccaagactgctcaagctcagcccactgcactctcatct
tgcaggggacaatagcaagttccgtgagggcaggttttcatgggtcacgg
ataacggcaagc

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