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GrainGenes Sequence Report: BG606586

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Sequence
BG606586
Contig
Ta.14516.2.S1_a_at
NSFT03P2_Contig16724
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC290932
wEST map position
BG606586
NCBI UniGene
Ta.14516
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus, strongly similar to NP_001055826.1 Os05g0474600 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Triticum aestivum
Cultivar
Brevor
Chromosome
1AL
1BL
1DL
Clone Library
Wheat dormant embryo cDNA library
Tissue
Seed embryo
Developmental Stage
Mature seed
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE2958_D03_H06ZS Wheat dormant embryo cDNA library Triticum aestivum cDNA clone WHE2958_D03_H06, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE2958_D03_H06
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown to seed maturity under conditions favoring seed dormancy (L. Dohery at K. Walker_Simmons lab, Washington State University, Pullman, WA). Embryos were cut from mature dormant seed (Doherty). Total RNA was prepared from these embryos, polyA was purified, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab at the University of California, Riverside (Chin, Fenton). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology
Ta.14516.2.S1_a_at749WHE2AFFY
DNA
tttgagattcagaaggcgtgatcatccatggcgagcgccaaggcgacgat
ggggcaggagaagcaagaccacttcgttctcaagagcgggcatgccatgc
cggccgtcgggctgggcacgtggagagctggctcggataccgcccactcc
gttcagacggccatcaccgaggctggatacaggcatgtggacacagcggc
tgaatacggagtagaaaaggaggtcggcaaagggcttaaggctgccatgg
aagctggaatcgacaggaaagatttgtttgtcacgtcaaaactatggtgc
acaaacttggtgcctgagagggtgcggccagcattagagaacacgctcaa
ggatttacagttggactacatcgatctttaccatatccattggccgttcc
ggctgaaagacggggcacaccagcctccggaagcaggggaggtgctggaa
ttcgacat

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