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GrainGenes Sequence Report: BG608352

Sequence	BG608352
Contig	TaAffx.120936.1.S1_at NSFT03P2_Contig13313
External Databases	Data at GenBank Data at EMBL Data at DDBJ
wEST map position	BG608352
DB Remark	Locus Source: Triticum monococcum
Keyword	EST
Species	Triticum monococcum
Cultivar	DV92
Chromosome	5AL 5DL
Clone Library	Triticum monococcum early reproductive apex cDNA library
Tissue	Early reproductive apex
Developmental Stage	Seven week-old plants
Data Source	genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title	WHE2498_D02_H04ZS Triticum monococcum early reproductive apex cDNA library Triticum monococcum cDNA clone WHE2498_D02_H04, mRNA sequence.
Strain	lab_host E. coli XLOLR
Clone	WHE2498_D02_H04
Remark	DB_xref: taxon:4568 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; The tissue, total RNA, and poly(A) RNA were prepared from apex at double-ridge stage to terminal-spikelet stage during transition from vegetative state to flower state, a cDNA library was made, and the cDNA clones were in vivo excised at the University of California, Davis (V. Echenique, B. Stamova , J. Dubcovsky). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; The tissue, total RNA, and poly(A) RNA were prepared from apex at double-ridge stage to terminal-spikelet stage during transition from vegetative state to flower state, a cDNA library was made, and the cDNA clones were in vivo excised at the University of California, Davis (V. Echenique, B. Stamova, J. Dubcovsky). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology	TaAffx.120936.1.S1_at 1094 WHE2AFFY TaAffx.82952.1.S1_at 74 WHE2AFFY TaAffx.82952.1.S1_at 68 WHE2AFFY Ta.3983.1.S1_at 72 WHE2AFFY TaAffx.109207.1.S1_at 64 WHE2AFFY
DNA	ggccagttcagctacttcaatgccacatctttcgttggcaatccaaacct gtgtggaccataccttggtccatgccgccctggcattgctgatgctggtc acaccaaccatggccatggagggctgtctagcactatcaagctgctcatt gtgttaggcttgcttctttgctccattatatttgctaccgccgcaattct gaaggcgcggtcattgaagaaagccagtgatgcacgtatgtggaaactca ctgcattccagcgccttgatttcacttgtgatgatgtgctggattccctg aaagaggagaacattattggcaaaggcggggctggaactgtgtacaaggg atcaatgcccaatggtgatcatgtggccgtgaagaggctctctgcaatgg tccgtggctcatcgcatgatcatggattctcagcggagatacaaacactt gggaggattcggcatcgccatattgtgcgcctgctaggcttctgctcaaa caatgagaccaacctgctggtgtatgagtatatgcccaatggtagtctcg gggagcttctccatggcaagaagggcgaacacctgcactgggacactc

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