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GrainGenes Sequence Report: NSFT03P2_Contig11817

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Sequence	BG608354
Contig	TaAffx.29583.1.A1_at NSFT03P2_Contig11817
External Databases	Data at GenBank Data at EMBL Data at DDBJ
wEST map position	BG608354
DB Remark	Locus Source: Triticum monococcum
Keyword	EST
Species	Triticum monococcum
Cultivar	DV92
Chromosome	2AS 2DS
Clone Library	Triticum monococcum early reproductive apex cDNA library
Tissue	Early reproductive apex
Developmental Stage	Seven week-old plants
Data Source	genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title	WHE2498_D04_H08ZS Triticum monococcum early reproductive apex cDNA library Triticum monococcum cDNA clone WHE2498_D04_H08, mRNA sequence.
Strain	lab_host E. coli XLOLR
Clone	WHE2498_D04_H08
Remark	DB_xref: taxon:4568 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; The tissue, total RNA, and poly(A) RNA were prepared from apex at double-ridge stage to terminal-spikelet stage during transition from vegetative state to flower state, a cDNA library was made, and the cDNA clones were in vivo excised at the University of California, Davis (V. Echenique, B. Stamova , J. Dubcovsky). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; The tissue, total RNA, and poly(A) RNA were prepared from apex at double-ridge stage to terminal-spikelet stage during transition from vegetative state to flower state, a cDNA library was made, and the cDNA clones were in vivo excised at the University of California, Davis (V. Echenique, B. Stamova, J. Dubcovsky). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology	TaAffx.29583.1.A1_at 983 WHE2AFFY TaAffx.29583.1.A1_at 323 WHE2AFFY
DNA	acttttgcaagcagcttgacaacagtgttgtgggtatacgctccacaatc agtaaactatcccagctcctgaaaagcatgatgaagagctctggcggcat ttagaggtcggaaccaaggttaacccacagttctatgcgttcagatggat taccctgctattgacacaggagtttaagttccgtgactgcatccacctgt gggatgcgttattaggtgatccagaggggccccaggccaccttgttaagg atctgctgtgcaatgctaattcttgttcgaagacggctcttggctggcga cttcactgcaaacctcaagcttctccagaattacccacccacaaacattg accacttgttgcatattgctaataaattgcgtgggctagttccctgctga atgatttgttctttttgtttccaatgttgtttttatggtagtccgctgtc cagagatgatgcattaaatctgtaaatgaatatccatacgctctttttga cccattgtttatcgtgtaatgtatgcctgttaaaaaaaaaaaaaaaaaaa

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