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GrainGenes Sequence Report: Ta.29585.2.S1_at

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Sequence
BG906546
Contig
Ta.29585.2.S1_at
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC362490
DB Remark
Locus Source: Triticum aestivum (bread wheat)
Keyword
EST
Species
Triticum aestivum
Cultivar
Thatcher Lr1
Clone Library
TaLr1
Tissue
Leaf tissue
Developmental Stage
14 Days old
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
TaLr1150E01R TaLr1 Triticum aestivum cDNA clone TaLr1150E01 5', mRNA sequence.
Strain
lab_host E. coli XLOLR
Clone
TaLr1150E01
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Dr. Sylvie Cloutier; Cereal Research Centre, Agriculture and Agri-food Canada; 195 Dafoe Rd, Winnipeg, MB, Canada R3T 2M9; Tel: (204) 983-2340; Fax: (204) 983-4604; Email: scloutier@em.agr.ca; was cloned directionally, not all sequences generated with reverse; primer were from the 5' end (same with forward primer and 3' end).; Average inset size is >2.2 kb; Plate: 150 row: E column: 01; Seq primer: M13 Reverse.
Locus Comment: Contact: Dr. Sylvie Cloutier; Cereal Research Centre, Agriculture and Agri-food Canada; 195 Dafoe Rd, Winnipeg, MB, Canada R3T 2M9; Tel: (204) 983-2340; Fax: (204) 983-4604; Email: scloutier@agr.gc.ca; was cloned directionally, not all sequences generated with reverse; primer were from the 5' end (same with forward primer and 3' end).; Average inset size is >2.2 kb; Plate: 150 row: E column: 01; Seq primer: M13 Reverse.
Note: Vector: Lambda ZapII; mass excised in plasmid vector pBK-CMV (Stratagene).; Site_1: EcoRI; Site_2: XhoI; mRNA obtained from wheat NIL Thatcher Lr1 24 hours after inoculation with leaf rust pathogen Puccinia triticina race BBB carrying the avirulence gene Avr1.
DNA
ggatcatgtacagacatattgtatgcaataggaatgtttcacatattacg
aagattattgtctgccaaaagaacaatgcaatatttcatataaagaagca
tcatctagatggtatattgcattttgctgatgaggagagagcatactcag
ttgatctatttgaagaaatgaggaaaatcaggtgacagttgatctattct
ttaagttatcacaatacatgatgcctcgtgccgaattcggcacgagctca
acaccaacgtcagcgcggcgacgagcctcctcacctggacctgcctcgac
gttatcttcttcggcaagccgttccgtcatcggcgccgtccagggcatga
tgaccggcctcgtctgcatcacccccggcgcatggctggtgcagacgtgg
gcggcggtggtgatgggcatcttctcg

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