GrainGenes Sequence Report: BG908437
Sequence
BG908437
Contig
Ta.4360.2.S1_at
External Databases
Data at GenBank Data at EMBL Data at DDBJ
NCBI UniGene
Ta.4360
DB Remark
Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, strongly similar to NP_001041991.1 Os01g0142300 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Triticum aestivum
Cultivar
Thatcher Lr1
Clone Library
TaLr1
Tissue
Leaf tissue
Developmental Stage
14 Days old
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
TaLr1168A07R TaLr1 Triticum aestivum cDNA clone TaLr1168A07 5', mRNA sequence.
Strain
lab_host E. coli XLOLR
Clone
TaLr1168A07
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Dr. Sylvie Cloutier; Cereal Research Centre, Agriculture and Agri-food Canada; 195 Dafoe Rd, Winnipeg, MB, Canada R3T 2M9; Tel: (204) 983-2340; Fax: (204) 983-4604; Email: scloutier@em.agr.ca; was cloned directionally, not all sequences generated with reverse; primer were from the 5' end (same with forward primer and 3' end).; Average inset size is >2.2 kb; Plate: 168 row: A column: 07; Seq primer: M13 Reverse. Locus Comment: Contact: Dr. Sylvie Cloutier; Cereal Research Centre, Agriculture and Agri-food Canada; 195 Dafoe Rd, Winnipeg, MB, Canada R3T 2M9; Tel: (204) 983-2340; Fax: (204) 983-4604; Email: scloutier@agr.gc.ca; was cloned directionally, not all sequences generated with reverse; primer were from the 5' end (same with forward primer and 3' end).; Average inset size is >2.2 kb; Plate: 168 row: A column: 07; Seq primer: M13 Reverse. Note: Vector: Lambda ZapII; mass excised in plasmid vector pBK-CMV (Stratagene).; Site_1: EcoRI; Site_2: XhoI; mRNA obtained from wheat NIL Thatcher Lr1 24 hours after inoculation with leaf rust pathogen Puccinia triticina race BBB carrying the avirulence gene Avr1.
DNA
ttagaattcggcacgagccccgccgtctttcttggtcccacacgcagcac
ccattccaacacacacacgcaacaattgcagcttgtaccagcacacagcc
aaggcaagattctgctaggtttggttcttggtttcttgcgggggcggcgg
caatggcgatcggcaccgacgacctggaggaggcgccgccgctcctgctc
ctcgacgacgacgaggccgcctacccgcgcccccgccgcgtcgcgctctt
cgtcgagccgtcgccattcgcctacatctctgggtacaagaatcggttcc
agaacttcatcaagcatctgcgcgaaatgggcgacgaggtcattgttgtg
accaaccatgagggggttccacaggagttccacggtgctaaggttattgg
ttcatggagctttccatgtccactctatggaaaggttcctctctcactgg
cactcagccctaggatcatctcagaggttgcaaagttcaagcctgacatc
attcatgcgtcctcacctggaatcatggtttttggagcccttgcgattgc
taaactgctcagtgtccccctggtgatgtcctaccacacccatgtcccag
tataca

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: BG908437
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