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GrainGenes Sequence Report: BI479113

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Sequence	BI479113
Contig	Ta.4874.1.S1_at NSFT03P2_Contig14679
External Databases	Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index	TC283308
wEST map position	BI479113
NCBI UniGene	Ta.4874
DB Remark	Locus Source: Triticum aestivum (bread wheat) UniGene title 'PMA1951'
Keyword	EST
Species	Triticum aestivum
Cultivar	Brevor
Chromosome	5AS 5BS 5DS
Clone Library	Wheat dormant embryo cDNA library
Tissue	Seed embryo
Developmental Stage	Mature seed
Data Source	genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title	WHE2952_F02_K04ZS Wheat dormant embryo cDNA library Triticum aestivum cDNA clone WHE2952_F02_K04, mRNA sequence.
Strain	lab_host E. coli SOLR
Clone	WHE2952_F02_K04
Remark	DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown to seed maturity under conditions favoring seed dormancy (L. Dohery at K. Walker_Simmons lab, Washington State University, Pullman, WA). Embryos were cut from mature dormant seed (Doherty). Total RNA was prepared from these embryos, polyA was purified, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab at the University of California, Riverside (Chin, Fenton). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology	Ta.4874.1.S1_at 900 WHE2AFFY Ta.4874.1.S1_at 289 WHE2AFFY TaAffx.31117.1.S1_at 139 WHE2AFFY TaAffx.31117.1.S1_at 137 WHE2AFFY Ta.5829.1.S1_at 84 WHE2AFFY
DNA	ggcagcaggcttgacgcgcgcacgctcaaggacgaggtggcgagcatgga ccggcgcccgctcctcgacctcggccacccgctcctcaaccgcgtcgccg acagcttcatccgcgccgccggagtcggagcggcgagggcggtgtcgcgg gaggcctacttcgtcgccgtcgaagggatgggaggagactcgacggggct ggacagcaccgccaagaggagccatttctccagcgccagaggggatgacg gccagaagtcgctcgacgcagtggttaaatcggcaagcaaggaggccatt cagtggggattggcagccggagtgtactctgggataacgtacggtctgag agaggccagaggacaccatgactggaagaacagtgcgatagcaggggcga tcgccggagcggcagtcgcgctgaccggggacaacgggcactccgaccac gtcgtccatttcgccatcaccggagccgcactgtcgagcgcggcgaccat gctctcaggcatattctaagccacg

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