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GrainGenes Sequence Report: BI479207

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Sequence
BI479207
Contig
NSFT03P2_Contig1373
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC365844
wEST map position
BI479207
NCBI UniGene
Ta.25511
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus, moderately similar to NP_001048888.1 Os03g0135600 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Triticum aestivum
Cultivar
Brevor
Chromosome
4BL
5AL
Clone Library
Wheat dormant embryo cDNA library
Tissue
Seed embryo
Developmental Stage
Mature seed
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE2954_B02_D04ZS Wheat dormant embryo cDNA library Triticum aestivum cDNA clone WHE2954_B02_D04, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE2954_B02_D04
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown to seed maturity under conditions favoring seed dormancy (L. Dohery at K. Walker_Simmons lab, Washington State University, Pullman, WA). Embryos were cut from mature dormant seed (Doherty). Total RNA was prepared from these embryos, polyA was purified, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab at the University of California, Riverside (Chin, Fenton). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology
Ta.25511.1.A1_at391WHE2AFFY
Ta.25511.1.A1_at272WHE2AFFY
Ta.25511.1.A1_at90WHE2AFFY
Ta.25511.2.S1_at270WHE2AFFY
DNA
acctgatctggaagtgctgcttctagtaagtggctgccacttcacaccct
tgctgcatcaggagacttctatctattggacaatcttctgaaacataagg
tcgatgtaaatgcacttgataaggacggtttgccagcaattcataaagca
attatttcgaagaagcatgctatcatcaactatctcttaaggaattcagc
aaatccatccgtctatgatagagatggtgcaaccctaatgcattatgctg
tccaaacagcatgcaatcagactataaaaactctattgctctacaatgtt
gatatcaatcgcccggatgattatggctggacaccgttgcatctagccgt
gcaaacacagagaaccgatattgtgaggctactcttactaaaaggatgga
ttgactcctttggacttgtgcctccgattgggtcataactcactgaagtt
tattactagtgttggactagctgagatgcaacttcctgctgcgcatgacg
gaattgcttccagttgcagataggacatgtttgctgctggagagaaatga
actacctgggttattttctatccacgaaaactggaaagcaaatcttttca
ca

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