GrainGenes Sequence Report: BI953342

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Sequence

BI953342

External Databases

Data at GenBank

Data at EMBL

Data at DDBJ

DB Remark

Locus Source: Hordeum vulgare subsp. vulgare

Keyword

EST

Species

Hordeum vulgare subsp. vulgare

Cultivar

Morex

Clone Library

Hordeum vulgare green seedling EST library HVcDNA0014 (Blumeria infected)

Tissue

green seedling leaf

Data Source

genbank	Release 135, Apr 15 2003
genbank	Updated Nov 2006

Title

HVSMEm0012E02f Hordeum vulgare green seedling EST library HVcDNA0014 (Blumeria infected) Hordeum vulgare subsp. vulgare cDNA clone HVSMEm0012E02f, mRNA sequence.

Strain

lab_host TJC121

vulgare

Clone

HVSMEm0012E02f

Probe

UMB502

Remark

DB_xref: taxon:112509

Feature: source: mol_type = 'mRNA';

Locus Comment: Contact: Wing RA; Clemson University Genomics Institute; Clemson University; 100 Jordan Hall, Clemson, SC 29634, USA; Tel: 864 656 7288; Fax: 864 656 4293; Email: rwing@clemson.edu; Total hq bases = 175; Seq primer: AATTAACCCTCACTAAAGGG; High quality sequence start: 2; High quality sequence stop: 794.

Note: Vector: pBluescript SK(-); Site_1: EcoRI; Site_2: XhoI; Morex (mla) plants were greenhouse grown in the R Wise lab at Iowa State University, Ames, IA; 7 day old green seedlings were infected with isolate 5874 of Blumeria graminis f. sp. hordei, and leaves were harvested 24, 48 and 72 hr post-inoculation and snap frozen (Wise). In the TJ Close lab at the University of California, Riverside, total RNA was prepared from each sample pool, equal quantities of all three RNA pools were combined, poly(A) RNA was purified from the mixture, one primary unamplified cDNA library was made, and 1 million pfu were in vivo excised to give pBluescript SK(-) cDNA phagemids (Chin). Phagemids were plated and picked at the Clemson University Genomics Institute (CUGI) (Begum, Palmer, Frisch, Atkins and Wing). Plasmid DNA preparations, DNA sequencing and sequence analysis were performed at CUGI (Wing, Yu, Frisch, Henry, Simmons, Oates, Rambo, Main). The sequence has been trimmed to remove vector sequence and contains a minimum of 100 bases of phred value 20 or above. For more details on library preparation and sequence analysis see http:

DNA

ggggggtgcttccttccaaacaagacaaacagactcgcattcgcatccca
tctccctcctcctaccgtaaaaaccaaaacaaaacaaccaaaccttcgac
tcgaaagagaagaaagaaagaaaggaacaagatggagagccatcaccggc
ggtcgagagtggggggggggaggtggagcgcaaggtgcagaagagctact
gggaggagcactccaaggacctcagcggggagtccatgaggctggagtcc
cgcgccaaggacctcgacaaggatgagcgcccagaggggcgtggggtcgt
cggggggtaggagggcgagactgtgctggagctcgggggcgggcagtggg
ggggggggcggggggctggcgcggggggcgggggaggggggggggcgggg
ggtggtggggggggggatgggggggagcgagggggggggtgggcgcgggg
ggggggggagggggggggggggcggcgcgggggggggggggggggggggg
gggggggggggggcggggggggggtggggggggggggggagggggggggg
agagccgggggaggcggggggaggggggggggcggggtgggtggggggga
ggggggggcggggcggggggggggcggggggcgggggggggggggggggg
ggggggagagggcggggggcggggggggggngtggtgcgcggtgcagggc
gggaggtgggatgccgggggggggcgggcggggggcgggggcagcggggg
gggcggcggggcgcggggcgcgggcggggggcgggggggggggggggggt
ggaggtgcgaggggggcggtcgggtgcggggggaaaagggggggcgggcg
cggggcgggggcg


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