GrainGenes Sequence Report: BI954579

HVSMEm0018L11f Hordeum vulgare green seedling EST library HVcDNA0014 (Blumeria infected) Hordeum vulgare subsp. vulgare cDNA clone HVSMEm0018L11f, mRNA sequence.

Strain

lab_host TJC121

vulgare

Clone

HVSMEm0018L11f

Probe

UMB009

Remark

DB_xref: taxon:112509

Feature: source: mol_type = 'mRNA';

Locus Comment: Contact: Wing RA; Clemson University Genomics Institute; Clemson University; 100 Jordan Hall, Clemson, SC 29634, USA; Tel: 864 656 7288; Fax: 864 656 4293; Email: rwing@clemson.edu; Total hq bases = 485; Seq primer: AATTAACCCTCACTAAAGGG; High quality sequence stop: 550.

Note: Vector: pBluescript SK(-); Site_1: EcoRI; Site_2: XhoI; Morex (mla) plants were greenhouse grown in the R Wise lab at Iowa State University, Ames, IA; 7 day old green seedlings were infected with isolate 5874 of Blumeria graminis f. sp. hordei, and leaves were harvested 24, 48 and 72 hr post-inoculation and snap frozen (Wise). In the TJ Close lab at the University of California, Riverside, total RNA was prepared from each sample pool, equal quantities of all three RNA pools were combined, poly(A) RNA was purified from the mixture, one primary unamplified cDNA library was made, and 1 million pfu were in vivo excised to give pBluescript SK(-) cDNA phagemids (Chin). Phagemids were plated and picked at the Clemson University Genomics Institute (CUGI) (Begum, Palmer, Frisch, Atkins and Wing). Plasmid DNA preparations, DNA sequencing and sequence analysis were performed at CUGI (Wing, Yu, Frisch, Henry, Simmons, Oates, Rambo, Main). The sequence has been trimmed to remove vector sequence and contains a minimum of 100 bases of phred value 20 or above. For more details on library preparation and sequence analysis see http:

DNA

cccatccccatccccatccagatccagccgtcgatgctgcagccccagcc
cacccgcatcgagctccgcagctccgaacgcgacgagatcgaggagcacc
tccgctccaaagccgccgccgccgccgccgccgccgccgaatcctcgcac
ctctcgaccccgccgacgtccaaccccatccccaacccgctcctccgcat
cctccacccgcacgccgacgccgcgcccaccaaggcccagcgcatcggcc
tccaccccccgccccccaaaccctagccccatggaggtcgaggtcaagct
ccgcctccccgacgcggccgcgcaccgccgcctctccgcctacctcgcgc
ctcgcctcctgcgcaccgacgcccagcgcaacgccttcttcgacgccccg
gcccggcccctcgccgccgccaccgccgcgctccgcgtccgcctctacgg
ccccgacgaccgcgctccctcccgcgccgtcctcgcgctcaagcgccgac
cgcgcatgcacgccggcgtcagccgcgtcaaggaggtcnaggagccgctc
gaaccggacctcgcccttncctgcgtcgacgacgcngccggtctgggcgc
gcccgactcacgctngacgagctcgtcgcagaggagtgcggcgcgagggg
g


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