GrainGenes Sequence Report: BM098137

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Sequence

BM098137

External Databases

TIGR Gene Index

NCBI UniGene

DB Remark

Locus Source: Hordeum vulgare subsp. vulgare

UniGene title 'CDNA clone: FLbaf44f08, mRNA sequence'

Keyword

EST

Species

Hordeum vulgare subsp. vulgare

Cultivar

Optic

Clone Library

pistil, 4 DPA, no treatment, cv Optic, EBpi03

Tissue

pistil

Developmental Stage

4 DPA

Data Source

genbank	Release 135, Apr 15 2003
genbank	Updated Nov 2006

Title

EBpi03_SQ002_K13_R pistil, 4 DPA, no treatment, cv Optic, EBpi03 Hordeum vulgare subsp. vulgare cDNA clone EBpi03_SQ002_K13 5', mRNA sequence.

Strain

lab_host DH10B

vulgare

Clone

EBpi03_SQ002_K13

Probe

kr18E0810

Remark

DB_xref: taxon:112509

Feature: source: mol_type = 'mRNA';

Locus Comment: Contact: Waugh R, Marshall DF; Genome Dynamics/Computational Biology; Scottish Crop Research Institute; Invergowrie, Dundee, DD2 5DA, Scotland, UK; Tel: 00 44 1382 562731; Fax: 00 44 1382 562426; Email: est@scri.sari.ac.uk; All sequence has a Phred quality score of 20 or over; Seq primer: M13 reverse.

Note: Vector: pSPORT1; Site_1: Sal I; Site_2: Not I; Non-normalised library, directionally cloned into pSPORT1. Derived from pistils dissected from developing grains (4 days post anthesis) in glasshouse grown barley plants. Developed as part of the barley transcriptome resources of BBSRC/SEERAD funded cereal IGF (Investigating Gene Function) project.

DNA

ctatggttaaggaagggcggccactctggcagcaaagaacttgatcaggg
atttatgttgaaacaatgaatatcgaatgatgatgctgcagactttgttt
gtttgccaacttgtgcaatttattgcaaaaaatggccagacttgataggc
atccaccagatcatcttttgttttgtgttttccatgtattgtcgttgctt
gggtagcactttctttggaatgttgatggaaaccgtcctggtattagtta
tgcgtggcatctctgggtttgttttgtgacgatttaagtttactttcgca
tgtccaagagtttcttccctctcaacgaatttggattgttttcagaactc
acagtatagcactttgttgactggtta


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