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GrainGenes Sequence Report: BM136927

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Sequence
BM136927
Contig
NSFT03P2_Contig989
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC291703
wEST map position
BM136927
NCBI UniGene
Ta.9534
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus, strongly similar to NP_001054289.1 Os04g0681400 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Triticum aestivum
Cultivar
Sumai3
Chromosome
2DL
Clone Library
Wheat Fusarium graminearum infected spike cDNA library
Tissue
Spike
Developmental Stage
Adult plant
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE0467-0470_I19_I19ZS Wheat Fusarium graminearum infected spike cDNA library Triticum aestivum cDNA clone WHE0467-0470_I19_I19, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE0467-0470_I19_I19
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20. No effort was taken; to identify ESTs of fungal origin from this library, thus this EST; could be of wheat or fungal origin.; Seq primer: Stratagene SK primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid pBluescript SK; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Spikes were sprayed at anthesis with Fusarium graminearum. Total RNA, and poly(A) RNA were prepared and pooled from infected spike at 0, 6, 12, 24, 36 and 48 hours after inoculation, a cDNA library was made , and the cDNA clones were in vivo excised to give pBluescript phagemids in G. Muehlbauer lab at the University of Minnesota (Kruger, W.M., Muehlbauer, G.J., Pritsch, C., Vance, C.). The cDNA library should contain genes of both wheat and fungal pathogen origin. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
Note: Vector: Lambda Uni-ZAP XR, excised phagemid pBluescript SK; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Spikes were sprayed at anthesis with Fusarium graminearum. Total RNA, and poly(A) RNA were prepared and pooled from infected spike at 0, 6, 12, 24, 36 and 48 hours after inoculation, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in G. Muehlbauer lab at the University of Minnesota (Kruger, W.M., Muehlbauer, G.J., Pritsch, C., Vance, C.). The cDNA library should contain genes of both wheat and fungal pathogen origin. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology
Ta.9534.1.A1_at293WHE2AFFY
TaAffx.31027.1.S1_at244WHE2AFFY
TaAffx.31027.1.S1_at198WHE2AFFY
DNA
gttgttattggtgaaagtgatccatacgtccacataagcgggcgggaaat
aagccatcttcatgttgccacagtgctgatgacagcaataatgatcattg
cttttctgtcctcgatagctattgtccggcgtatactgatagccacacct
gtcctgaagttgctgcgaaggtcattggtgaagttcaggcactgatagtt
ttcccagttgtgccgtattttatcctcgctatcttttatatgttctggtt
ttctgctctactccacctcttcagctctggtcaagttgtccgaaatgact
gcagtacagattgttgttcatatgatctgaagttgggcaaagtgaactgt
gacaactgttgtgggtacagcatccactacacccct

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