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GrainGenes Sequence Report: BQ159692

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Sequence	BQ159692
External Databases	Data at GenBank Data at EMBL Data at DDBJ
wEST map position	BQ159692
DB Remark	Locus Source: Aegilops speltoides
Keyword	EST
Species	Aegilops speltoides
Cultivar	F2 from 2-12-4-8-1-1-1-(1) x PI36909-12-811-(1 ) F2 from 2-12-4-8-1-1-1-(1) x PI36909-12-811-(1)
Chromosome	2BS 6AS 6BS
Clone Library	Aegilops speltoides anther cDNA library
Tissue	Anther
Developmental Stage	Premeiotic anthers
Data Source	genbank Release 135, Apr 15 2003 genbank Downloaded 2008-2009
Title	WHE2222_F04_K08ZT Aegilops speltoides anther cDNA library Aegilops speltoides cDNA clone WHE2222_F04_K08, mRNA sequence.
Strain	lab_host E. coli SOLR
Clone	WHE2222_F04_K08
Probe	WHE2222_F04_K08
Remark	DB_xref: taxon:4573 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in a growth chamber at the University of California, Davis (Akhunov). Premeiotic anthers were harvested, total RNA and poly(A) RNA were prepared, from each tissue and then pooled, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Akhunov, Chin, Choi, Close, Fenton, Kianian, Otto, Simons , Zhang) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in a growth chamber at the University of California, Davis (Akhunov). Premeiotic anthers were harvested, total RNA and poly(A) RNA were prepared, from each tissue and then pooled, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Akhunov, Chin, Choi, Close, Fenton, Kianian, Otto, Simons, Zhang) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA	ttttttttttttttttttttttttccccagtacaaatacagggcattaga atttctgtttacatagtgatatacaagtaggctttcaacaaaaatgcaac tagcctactacttcaactattcagtttcaagatcagattcccnctagaag agtatcactcagaaggccatccaaaactacaagagataaataaacgaaag ctgatacaaactactagtcagaaacggaaacactactcccagggaatttc gagacttcatgtcatcttcatggccaaaataagaacattctttcatgaaa ggcagatggaggattgcattgaatactgcaatggccattttagaacacaa agatacagagtgtgcagaaatactactcattgatacttcaaagaaattgg cagaatgccctgacctcatactctcctagttccaagatgtgtgcaatatg acaacacgaggaaggataagttcatagcagtgcaatgctttcttctctac tgcatgacataatgaacataccaattttcttattagtcttggttcatgtg aaactgaatctagctggaacctctcctctgcaagctgctccatagtcatt a

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