GrainGenes Sequence Report: BQ160147

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Sequence

BQ160147

Contig

NSFT03P2_Contig16477

External Databases

Data at GenBank

Data at EMBL

Data at DDBJ

wEST map position

BQ160147

DB Remark

Locus Source: Secale cereale (rye)

Keyword

EST

Species

Secale cereale

Cultivar

Blanco

Chromosome

2AS

2BS

2DS

Clone Library

Secale cereale anther cDNA library

Tissue

Anther

Developmental Stage

Adult plant before anthesis

Data Source

genbank	Release 135, Apr 15 2003
genbank	Downloaded 2008-2009

Title

WHE1272_H12_O24ZT Secale cereale anther cDNA library Secale cereale cDNA clone WHE1272_H12_O24, mRNA sequence.

Strain

lab_host E. coli SOLR

Clone

WHE1272_H12_O24

Probe

WHE1272_H12_O24

Remark

DB_xref: taxon:4550

Feature: source: mol_type = 'mRNA';

Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer.

Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Anthers were harvested and pooled from early meiosis to late meiosis. The tissue, total RNA, and poly(A) RNA were prepared (Butler, Ross and Gustafson) at University of Missouri, Columbia. A cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

DNA

ttttttttttttttttttttttttcaaaattaactttgctgatgaaatca
atcattttggtaagagggtgtaaacaccctaatctggcaacaccataaaa
caagcacgccagaatcaatacacggcaagcccaactatggcaatacaagg
gtagcaattattccccagcttatatcacaggcgggtagcaggctgacgaa
tagaaagtttataaattctcatctcctgttatcaagagaggaaatgggcc
accaacttacaagaggaacatttgtttgctaaaaatgagactagcacccc
tcctgcaccgcacaattcgacttccccatgctggaacaactgaacaaggc
tgtaatgtaaagcagtgactgtgcttcagaatggccatgacatttgaacc
gtgtcatcacgggatcatagtgtatgttcgccacaggatcagtatccgtt
aatctttcgctgtga


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