GrainGenes Sequence Report: BQ160494
Sequence
BQ160494
Contig
NSFT03P2_Contig17903
External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC4300
wEST map position
BQ160494
DB Remark
Locus Source: Secale cereale (rye)
Keyword
EST
Species
Secale cereale
Cultivar
Blanco
Chromosome
5DL
Clone Library
Secale cereale anther cDNA library
Tissue
Anther
Developmental Stage
Adult plant before anthesis
Data Source
genbank Release 135, Apr 15 2003 genbank Downloaded 2008-2009
Title
WHE1821-1824_M09_M09ZT Secale cereale anther cDNA library Secale cereale cDNA clone WHE1821-1824_M09_M09, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE1821-1824_M09_M09
Probe
WHE1821-1824_M09_M09
Remark
DB_xref: taxon:4550 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Anthers were harvested and pooled from early meiosis to late meiosis. The tissue, total RNA, and poly(A) RNA were prepared (Butler, Ross and Gustafson) at University of Missouri, Columbia. A cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
ttttttttttttttttttctgaactttctaatgcattgaagagatgaact
tggaacaattcttacatctcacattacaggcccttggctcactgaccacg
caatcccatcatggcttgctactttctgaatcacacaagaaagcaacaaa
acaaccacttacagctactggtagctttagcctaagctcacattgctcat
caatctcacagaaacctcaggcttactgctaacaaaactagtggcctcct
cgtccttgcaggggaaggttacatatgtagtatgttcagaacagcacgac
gagggcgatgacactagccatggcaataagcataccgatgctcgccttca
gcccagcagcgccgttcaagccccagacgcggacgtcgtcgatcaccggc
ccgcagagcgagctgtggtcgtcgcttctcgtgttgtagtagacgctgt
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: BQ160494
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||