GrainGenes Sequence Report: BQ160642
Sequence
BQ160642
External Databases
Data at GenBank Data at EMBL Data at DDBJ
wEST map position
BQ160642
DB Remark
Locus Source: Secale cereale (rye)
Keyword
EST
Species
Secale cereale
Cultivar
Blanco
Chromosome
7AL 7DL
Clone Library
Secale cereale anther cDNA library
Tissue
Anther
Developmental Stage
Adult plant before anthesis
Data Source
genbank Release 135, Apr 15 2003 genbank Downloaded 2008-2009
Title
WHE1256_G03_M06ZY Secale cereale anther cDNA library Secale cereale cDNA clone WHE1256_G03_M06, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE1256_G03_M06
Probe
WHE1256_G03_M06
Remark
DB_xref: taxon:4550 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: Oligo dT wobble primer (an equal mixture of (T)27A, (T; )27G and (T)27C). Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: Oligo dT wobble primer (an equal mixture of (T)27A,; (T)27G and (T)27C). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Anthers were harvested and pooled from early meiosis to late meiosis. The tissue, total RNA, and poly(A) RNA were prepared (Butler, Ross and Gustafson) at University of Missouri, Columbia. A cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
caacacaacggcaaaaaaaggaaaaactgttcatggatcgccacacaaac
aacaatcaccccgtcccattctgggagctctctaataatccgagaaaaac
tgaagctgcctgcaccggtggtgcttcttgacttgcatcc
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: BQ160642
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||