GrainGenes Sequence Report: NSFT03P2_Contig5704
Sequence
BQ160652
Contig
NSFT03P2_Contig5704
External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC4957
wEST map position
BQ160652
DB Remark
Locus Source: Secale cereale (rye)
Keyword
EST
Species
Secale cereale
Cultivar
Blanco
Chromosome
6BS 6DS
Clone Library
Secale cereale anther cDNA library
Tissue
Anther
Developmental Stage
Adult plant before anthesis
Data Source
genbank Release 135, Apr 15 2003 genbank Downloaded 2008-2009
Title
WHE1803_F03_L05ZY Secale cereale anther cDNA library Secale cereale cDNA clone WHE1803_F03_L05, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE1803_F03_L05
Probe
WHE1803_F03_L05
Remark
DB_xref: taxon:4550 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: Oligo dT wobble primer (an equal mixture of (T)27A, (T; )27G and (T)27C). Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: Oligo dT wobble primer (an equal mixture of (T)27A,; (T)27G and (T)27C). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Anthers were harvested and pooled from early meiosis to late meiosis. The tissue, total RNA, and poly(A) RNA were prepared (Butler, Ross and Gustafson) at University of Missouri, Columbia. A cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
aacatctcaggctttctcctaaatcacaacaggctgataaaatcatgaca
gatatgaaatccataaaatcgatgtcactatcccaaggacagtcagaaaa
tcgatcgtcagccctcaaggttcagagttacactgactaatcgctactac
agatcacgttgggcgggtagatcaacctcacgcagcagccgacttgcgca
gctcgtttacgccgtgggcaaagtggcatctgtcgccaaacgtgcacgat
cctttcgtgaagttgtcacacagcttggtcttgaagttgcttcctggccc
accaccgccgccacgggtgagatcctccagccgggatcacgcccgggcgg
aggggcattggcggcggcgccgctgatcctgaagatcaactccctgagca
tggcgctggcgttgttgatctgatcaaacgtgccctcaagctcaatgttt
ttcaaggcttcgtttgattcatggtcccggatggccagcttggccccggt
gactcggg
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: NSFT03P2_Contig5704
|
| ||||||
|
| ||||||
|
| ||||||
|
| ||||||
|
| ||||||
|
| ||||||
|
| ||||||
|
| ||||||
|
| ||||||
|
| ||||||
|
| ||||||
|
| ||||||
|
| ||||||
|
| ||||||
|
| ||||||
|
| ||||||
|
| ||||||
|
| ||||||
|
| ||||||
|
|
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||