GrainGenes Sequence Report: BQ160713

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Sequence

BQ160713

Contig

Ta.7803.1.S1_at

NSFT03P2_Contig11608

External Databases

Data at GenBank

Data at EMBL

Data at DDBJ

wEST map position

BQ160713

NCBI UniGene

Ta.7803

DB Remark

Locus Source: Triticum aestivum (bread wheat)

UniGene title 'Transcribed locus, strongly similar to NP_001050053.1 Os03g0338000 [Oryza sativa (japonica cultivar-group)]'

Keyword

EST

Species

Triticum aestivum

Cultivar

Chinese Spring

Chromosome

4AS

4DL

Clone Library

Wheat drought-stressed seedling cDNA library

Tissue

Seedling without endosperm

Developmental Stage

Five day old seedling

Data Source

genbank	Release 135, Apr 15 2003
genbank	Updated Nov 2006

Title

WHE0286_B11_D22ZT Wheat drought-stressed seedling cDNA library Triticum aestivum cDNA clone WHE0286_B11_D22, mRNA sequence.

Strain

lab_host E. coli SOLR

Clone

WHE0286_B11_D22

Probe

WHE0286_B11_D22

Remark

DB_xref: taxon:4565

Feature: source: mol_type = 'mRNA';

Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer.

Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized , germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Five-day old seedlings were incubated for one day at 90% RH. After removing endosperm, seedlings were transferred to desiccator jar containing saturated MgSO4 at room temperature for 24 hr. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized, germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Five-day old seedlings were incubated for one day at 90% RH. After removing endosperm, seedlings were transferred to desiccator jar containing saturated MgSO4 at room temperature for 24 hr. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

DNA

tttttttttttttttttttgatggagagaggcggtaatatatttcaacaa
agtaaaggtcttatacaagttgcatcaggccaaatttcgtctgcttcact
acacgacgtaaggtaaacacagaagtaaactgcggctacttccgttaaac
tcaggtggaaagttgagttgcttcgacatggcagggagcccatcgcaagt
gctggttgggaacctcagagctttgacagcgctatgtccatcacatcgac
gaagaaatcagcatcttccttggtgaagcacagcgggggagtgattctga
acacgttgccgtaaaagccccctttccgaccaaaacgcccatgtctttca
tgtgctccatggcctggcaaatctcgtctttggccggggtcttgagctgc
cggtcggtcacaagctccacgccaagcatgaagcctgctcccctca


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