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GrainGenes Sequence Report: Ta.7805.1.S1_at

Sequence	BQ160715
Contig	Ta.7805.1.S1_at NSFT03P2_Contig11609
External Databases	Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index	TC287966
wEST map position	BQ160715
NCBI UniGene	Ta.7805
DB Remark	Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, moderately similar to NP_001062361.1 Os08g0536100 [Oryza sativa (japonica cultivar-group)]'
Keyword	EST
Species	Triticum aestivum
Cultivar	Chinese Spring
Chromosome	7AS 7BS 7DS
Clone Library	Wheat drought-stressed seedling cDNA library
Tissue	Seedling without endosperm
Developmental Stage	Five day old seedling
Data Source	genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title	WHE0286_C03_F06ZT Wheat drought-stressed seedling cDNA library Triticum aestivum cDNA clone WHE0286_C03_F06, mRNA sequence.
Strain	lab_host E. coli SOLR
Clone	WHE0286_C03_F06
Probe	WHE0286_C03_F06
Remark	DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized , germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Five-day old seedlings were incubated for one day at 90% RH. After removing endosperm, seedlings were transferred to desiccator jar containing saturated MgSO4 at room temperature for 24 hr. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized, germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Five-day old seedlings were incubated for one day at 90% RH. After removing endosperm, seedlings were transferred to desiccator jar containing saturated MgSO4 at room temperature for 24 hr. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA	ttttttttttttttttttaacaactaaaatgaggcattttattggaagat aaaacctgtacattttggggactggtggcaaattagtggaataaatatgt acagagttttcgcttatcggtgcgccgcagcacaggctatacatgacagc aaaagcaatttttcagttgcttatgtcgaagtacttgggatcagcaggga agtcatgttccaccttcagctcgccttcatccgcatctgagtgcctctga ggaaatacaactctaagatcgttgtagagatagaatctcctatcctcagt caccatattgccgctctgtggtacagatgatggatcggatttgcaacgta gcatagattttgaggattttttcgaggcggggcagagaaacgcaggtgca aagcatagcggagcacgcctccacctgaactgtcattgatcctgggaaga cgcacaacatgatttcttcgcacctgagtgcacgttgtccatctgacagc acaatcatcagaatcagtattgttttgcttattc

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