GrainGenes Sequence Report: NSFT03P2_Contig14975
Sequence
BQ160776
Contig
Ta.14166.1.S1_at NSFT03P2_Contig14975
External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC340131
wEST map position
BQ160776
NCBI UniGene
Ta.14166
DB Remark
Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, moderately similar to NP_001064934.1 Os10g0492800 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
1AL 1DL 5BL
Clone Library
Wheat unstressed seedling shoot cDNA library
Tissue
Etiolated shoot
Developmental Stage
Five day old seedling
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
WHE0326_H08_O16ZT Wheat unstressed seedling shoot cDNA library Triticum aestivum cDNA clone WHE0326_H08_O16, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE0326_H08_O16
Probe
WHE0326_H08_O16
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized , germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Shoots were harvested. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized, germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Shoots were harvested. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
ttttttattttttttttttttttttttttaagggcttctgcatgtcacta
tctttgcagcgaggctgcttacaagcatctttacattattttttcagcat
acatccaacacaagggggccattcgttgcccaagagttgtgagttgtagc
aaacgaaacaacaaaaatacaaaaagggaagaaagaaagaaaaaaaaacc
atgtacatgtaaaatgtcaatatctccttttttctttttctctgtttgat
gcagatttcaacgtcgtaccggacagctcaaccggattcagtttgtttgg
caaaaacagggggtccgtagtactttgaaaaatatttggcaacccacggc
gcaagctgagtgttctcaggctcccttctgttcttgttgtaatagtcaga
ccatatagctgggtatatttcagggttgaagccatcacgatatacacaaa
acggcaaccatccctggcctccgtttatccttcgctttctttctcgaggg
tatg
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: NSFT03P2_Contig14975
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||