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GrainGenes Sequence Report: BQ160987

Sequence	BQ160987
Contig	Ta.3233.1.A1_at Ta.3233.2.A1_a_at NSFT03P2_Contig9182
External Databases	Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index	TC317294
wEST map position	BQ160987
NCBI UniGene	Ta.3233
DB Remark	Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, weakly similar to NP_001132609.1 hypothetical protein LOC100194082 [Zea mays]'
Keyword	EST
Species	Triticum aestivum
Cultivar	Chinese Spring
Chromosome	4AL 5DL 6AL
Clone Library	Wheat unstressed seedling shoot normalized cDNA library
Tissue	Etiolated shoot
Developmental Stage	Five day old seedling
Data Source	genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title	WHE1060_B12_C24ZT Wheat unstressed seedling shoot normalized cDNA library Triticum aestivum cDNA clone WHE1060_B12_C24, mRNA sequence.
Strain	lab_host E. coli DH10B
Clone	WHE1060_B12_C24
Probe	WHE1060_B12_C24
Remark	DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7. Note: Vector: Lambda Uni-ZAP XR, excised phagemid pBluescript SK; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized, germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Roots were harvested. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. The cDNA clones were in vivo excised to give pBluescript phagemids before normalization was carried out. The mass excision of phagemid library and normalization were done in HT Nguyen lab by D. Zhang at Texas Tech Univeristy. Normalization protocol used was that of Soares'. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA	ttttttttttttttttatgttcataactaaatccgataatttattctttc ctatcatttgcctgaaaagttttacaagtctccaattgtacttccgatcc atcatatctgtaactcccccacaaaaagttacctacaccagacagttata aacttattggagacgacaacaagagtcgagcccgcccgcaatacaggcgg gcctttttttaactgatacaacccccgcggcagaggggcgctgctgcact atgtgccataacagacttgtgctcaacgcacagccacctgagttcagact tggcaacacacagtcatcaccatataactaacagtaacagtcacaaaaaa gacatttacaacagaagcagtcatctccacgtcgcgccaaagtgcgagac gaagaaccggaagtccgggtggtccacctgtacgcagcggagccagttgt ccgcctcctgcatcagcgacgtgacgctctcctgctccggggcgccgttg gacgcccacaaggagctgcggagcttgtaggacgccagcccgaaggcggg cagggaaagcctgcccgttgcggtggtgcagtggccgattccctcgaagc cggggcatgctggtgtggtgggat

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