GrainGenes Sequence Report: BQ161208
Sequence
BQ161208
Contig
Ta.8060.1.S1_at
External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC354217
wEST map position
BQ161208
NCBI UniGene
Ta.8060
DB Remark
Locus Source: Triticum aestivum (bread wheat) UniGene title 'Clone wlsu2.pk0001.h3:fis, full insert mRNA sequence'
Keyword
EST
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
7AS 7BS 7DS
Clone Library
Wheat cold-stressed seedling cDNA library
Tissue
Seedling
Developmental Stage
Five-day old seedling
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
WHE0366_G04_N08ZT Wheat cold-stressed seedling cDNA library Triticum aestivum cDNA clone WHE0366_G04_N08, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE0366_G04_N08
Probe
WHE0366_G04_N08
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized , germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Five-day old seedlings were transferred to 5 C cold room and kept for 48 hr. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized, germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Five-day old seedlings were transferred to 5 C cold room and kept for 48 hr. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
ttttttttttttttttggtcggccaaccaagatattttgaaagatatact
attccaacaaaatgatcttgattgaatttgattctcagtccttggacgtt
acactcttctacataaccaagtgtccggatttcctggtaaatatgaggcc
tgcactatatcatcaaacgttcaaagccatgatctcatgcatgggagtga
tcagaaacatcagtgcgagctaccgaaggaggggtaagctctccactcgt
gctgacctgacgtcatcgcagtcacaacatgatcttcctagctaaggcac
actattgttgttttgtcaagaagatcctgttgacaacatctaaa
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: BQ161208
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||