GrainGenes Sequence Report: BQ161347
Sequence
BQ161347
Contig
Ta.8115.1.S1_s_at NSFT03P2_Contig10960
External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC301374
wEST map position
BQ161347
NCBI UniGene
Ta.8115
DB Remark
Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, weakly similar to NP_001063552.1 Os09g0495200 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
5AL 5BL 5DL
Clone Library
Wheat etiolated seedling root normalized cDNA library
Tissue
Root
Developmental Stage
Five day old etiolated seedling
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
WHE1107_A12_B23ZT Wheat etiolated seedling root normalized cDNA library Triticum aestivum cDNA clone WHE1107_A12_B23, mRNA sequence.
Strain
lab_host E. coli DH10B
Clone
WHE1107_A12_B23
Probe
WHE1107_A12_B23
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid pBluescript SK; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized, germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Roots were harvested. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. The cDNA clones were in vivo excised to give pBluescript phagemids before normalization was carried out. The mass excision of phagemid library and normalization were done in HT Nguyen lab by D. Zhang at Texas Tech Univeristy. Normalization protocol used was that of Soares. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
tttttttttttttttttttgtgcatgaagaatgccacaaaactgtacaca
accatgggcaagtaaactagcattacataaatgttattacacattatact
aaaaattcttatataaaaatctcacataaacagatccacacaatctcagg
ggtctacattttatttcctacggaagcaatcgtcatatggagatgtatag
ctcggataaaacattattctgaacaattcttgagctccgcccttcattct
caataaattaactgtgagaatcagataatgctttgcatatttcttcatgc
gtgccacatcaaaaatgaatgtcgaaagataaagaaagaaaacaggcatc
ttgacaatatccaagagttcatagttcttcctgaagtccttggtagccat
aataaccacaataccaaatttcaaacgagtcaaccaatcattgcatttgc
cctgccggcgcacctccgaggacacaacgctcagtttatagaatgcatag
gcaagggcagcatcgacaaagatggaaggagcagccttccacagctggta
gcacccgacgcagccaagaaaagcaccaaacagcatgccgacttgataat
cctcgaggtcgaagacggtgcgaagggatcggatatccgagatgttgcgc
gccaggggcctgaacatgtcgcataccatggccttcagcgcccccggcgc
cccggttcgatctgaatgctggtcacccttcagaggaggggaatcaggcg
agcacccgcacgcccatggag
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: BQ161347
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||