GrainGenes Sequence Report: NSFT03P2_Contig11663
Sequence
BQ161784
Contig
Ta.8357.1.S1_at NSFT03P2_Contig11663
External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC323563
wEST map position
BQ161784
NCBI UniGene
Ta.10306
DB Remark
Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, weakly similar to NP_001052773.1 Os04g0418800 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Triticum aestivum
Cultivar
Sumai3
Chromosome
5AS 5DS
Clone Library
Wheat Fusarium graminearum infected spike cDNA library
Tissue
Spike
Developmental Stage
Adult plant
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
WHE0476_F12_K24ZT Wheat Fusarium graminearum infected spike cDNA library Triticum aestivum cDNA clone WHE0476_F12_K24, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE0476_F12_K24
Probe
WHE0476_F12_K24
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20. No effort was taken; to identify ESTs of fungal origin from this library, thus this EST; could be of wheat or fungal origin.; Seq primer: T7 primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid pBluescript SK; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Spikes were sprayed at anthesis with Fusarium graminearum. Total RNA, and poly(A) RNA were prepared and pooled from infected spike at 0, 6, 12, 24, 36 and 48 hours after inoculation, a cDNA library was made , and the cDNA clones were in vivo excised to give pBluescript phagemids in G. Muehlbauer lab at the University of Minnesota (Kruger, W.M., Muehlbauer, G.J., Pritsch, C., Vance, C.). The cDNA library should contain genes of both wheat and fungal pathogen origin. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors). Note: Vector: Lambda Uni-ZAP XR, excised phagemid pBluescript SK; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Spikes were sprayed at anthesis with Fusarium graminearum. Total RNA, and poly(A) RNA were prepared and pooled from infected spike at 0, 6, 12, 24, 36 and 48 hours after inoculation, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in G. Muehlbauer lab at the University of Minnesota (Kruger, W.M., Muehlbauer, G.J., Pritsch, C., Vance, C.). The cDNA library should contain genes of both wheat and fungal pathogen origin. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
tttttttttttttttttgaaagaactcatgctcgtgttgaagttaaacag
cggaggacacacgacagattcagtagacaatgctacgttttacagtaaca
ccacaacggtagcaaaacccacatgcatgattttttcgacaaagacccac
gggcatgatagcacgctaactctggacgaacacaaatcagcagcgggtaa
ccaagcaacctgcattattattcagactacatattacttaatctccaatg
agaataatggccctgcatgcgttggcagggactcgcagtcacagccctag
acattgggcttgtaggccggcggcgtcgatggcggctcctcatagtcggc
ggcgtcggcggcggcctgtacgccagcggagtcggcttccgcgccgtgct
cgaggaagggagatggcatcggggccggggcgtatgggggtggcgtgggt
tcaggaggggacggaatgtatgggggcggagtgggctcaggaggggacgg
agtgtacgggggcggcgttggcgtgggaggtgt
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: NSFT03P2_Contig11663
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||