GrainGenes Sequence Report: NSFT03P2

GrainGenes Sequence Report: NSFT03P2_Contig11620

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Sequence

BQ162092

Contig

Ta.8587.1.A1_at

NSFT03P2_Contig11620

External Databases

TIGR Gene Index

wEST map position

BQ162092

NCBI UniGene

Ta.8587

DB Remark

Locus Source: Triticum aestivum (bread wheat)

UniGene title 'Transcribed locus, moderately similar to NP_001063964.1 Os09g0567700 [Oryza sativa (japonica cultivar-group)]'

Keyword

EST

Species

Triticum aestivum

Cultivar

Sumai3

Chromosome

4BS

4DS

Clone Library

Wheat Fusarium graminearum infected spike cDNA library

Tissue

Spike

Developmental Stage

Adult plant

Data Source

genbank	Release 135, Apr 15 2003
genbank	Updated Nov 2006

Title

WHE0496_B04_C08ZT Wheat Fusarium graminearum infected spike cDNA library Triticum aestivum cDNA clone WHE0496_B04_C08, mRNA sequence.

Strain

lab_host E. coli SOLR

Clone

WHE0496_B04_C08

Probe

WHE0496_B04_C08

Remark

DB_xref: taxon:4565

Feature: source: mol_type = 'mRNA';

Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20. No effort was taken; to identify ESTs of fungal origin from this library, thus this EST; could be of wheat or fungal origin.; Seq primer: T7 primer.

Note: Vector: Lambda Uni-ZAP XR, excised phagemid pBluescript SK; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Spikes were sprayed at anthesis with Fusarium graminearum. Total RNA, and poly(A) RNA were prepared and pooled from infected spike at 0, 6, 12, 24, 36 and 48 hours after inoculation, a cDNA library was made , and the cDNA clones were in vivo excised to give pBluescript phagemids in G. Muehlbauer lab at the University of Minnesota (Kruger, W.M., Muehlbauer, G.J., Pritsch, C., Vance, C.). The cDNA library should contain genes of both wheat and fungal pathogen origin. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

Note: Vector: Lambda Uni-ZAP XR, excised phagemid pBluescript SK; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Spikes were sprayed at anthesis with Fusarium graminearum. Total RNA, and poly(A) RNA were prepared and pooled from infected spike at 0, 6, 12, 24, 36 and 48 hours after inoculation, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in G. Muehlbauer lab at the University of Minnesota (Kruger, W.M., Muehlbauer, G.J., Pritsch, C., Vance, C.). The cDNA library should contain genes of both wheat and fungal pathogen origin. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

DNA

ttttttttttttttttttaaaaagaaacggcattgcgcctatggacccaa
aaattcctcaagacctacggataaaactgttacagaatcatcaggcgacc
tcgctttctttctactgttgttacactcgcgatacagcgaacaggttaaa
gtcgttccgccgaggtcacaccttgacgaacaggcgggggacactaggaa
ctacaagcagaataaaacaaccacgggaaaagttctcaaaatagatgcta
tggtgtatgcacttttctctatgcttaccaaggggtcaataaatcgccag
caaaccggaccggaagggtttgttcgctatgccgcggtgtccttggcttc
tgtcggtgtcacgtccattttatcgaccttggcatctgctgctggaggtg
tcacgtccattttatcgaccttggcttctgctgctggaggtgtcacgtcc
attttgtcgacgttttcctcggcgacgctttcgtactccgacagcaggtc
catgaactcgttgagcagccgctggactttgcggtttgtcgccatcgaag
gaagtatgtcttccctgagaagtttatgtttcttcattccaagaacatca
ggatcccatgacggattattcgcatcggtagacacatcaccaaccatgcc
catgggagggccaagaaagctctcgcagacttcacgtaggcgagattcat
ctgcctctcttgccaaaaactaatgt


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