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GrainGenes Sequence Report: BQ162199

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Sequence
BQ162199
Contig
Ta.8669.1.S1_at
Ta.8669.2.S1_a_at
NSFT03P2_Contig10233
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC291416
wEST map position
BQ162199
NCBI UniGene
Ta.39535
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus, moderately similar to NP_001063753.1 Os09g0531100 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
1DL
Clone Library
Wheat heat-stressed seedling cDNA library
Tissue
Leaf
Developmental Stage
Two-week old seedling
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE0751_E11_I21ZT Wheat heat-stressed seedling cDNA library Triticum aestivum cDNA clone WHE0751_E11_I21, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE0751_E11_I21
Probe
WHE0751_E11_I21
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Two-week old seedling were heat treated at 37 C for 2 hr and cyclic heat treated at 37 C for 2 hr for two days at Texas Tech University (D. Zhang, H. Nguyen lab). The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
ttttttttttttttttttttggtggcgtgcgtttgtaccgtgttgttgtg
gattgtgctttatatataaagcagggcgaaagcctttttcagtaactaca
aatgtaccataaacgttacacagctgggccttgaagccggttcactattt
cctctgctctagcttgtatctcttttatatattcgacaagtacccgttgc
tgataagaacctccttccgtgcgaatcaagattctctcaattctcttctt
ggtaacagcgtaaaattcttcaggtgaaacatctccctttccaactgtaa
gtccatcaagcaacagcctattccagtcattttcgggagcctctattatc
gattcaagaaacttttcaggcactacaacctctccccctttataagcgta
actgcgctttcggagaaagttggaagcatatagttgcaacaccttttgaa
gcattacttgaagccctggcttatctacttcgtctttagcttgcctcagc
tga

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