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GrainGenes Sequence Report: BQ162303

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Sequence	BQ162303
Contig	Ta.8737.1.A1_at NSFT03P2_Contig13953
External Databases	Data at GenBank Data at EMBL Data at DDBJ
NCBI UniGene	Ta.66235
DB Remark	Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus'
Keyword	EST
Species	Triticum aestivum
Cultivar	Chinese Spring
Clone Library	Wheat heat-stressed seedling cDNA library
Tissue	Leaf
Developmental Stage	Two-week old seedling
Data Source	genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title	WHE0764_C06_E12ZT Wheat heat-stressed seedling cDNA library Triticum aestivum cDNA clone WHE0764_C06_E12, mRNA sequence.
Strain	lab_host E. coli SOLR
Clone	WHE0764_C06_E12
Remark	DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Two-week old seedling were heat treated at 37 C for 2 hr and cyclic heat treated at 37 C for 2 hr for two days at Texas Tech University (D. Zhang, H. Nguyen lab). The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA	ttttttttttttttttttacaaattagatattaatagtttctcaaaacaa attacatataggaataaccacagttaagataacacggtgcaaaactccct aatagagaaccataactccctccaggatcaggatacagcatgtggtaaaa cagatgccagggattacagaaacttgagcaagatagcattccacaaagat ctattcctaataatccaaaggcgatgacggcactcgctagtgatggtgct gaagagatccttcgtcttgctgggccgaagaagcatccattgtgtgaagg ggagcacctgagacattgatcaccttaccgaagcgctcggaggtaagttg cgcccactcacagacccctggtaaaattttgtgcaggtcagtattatcac ccagatatcttgtgcctgtgattcttggttctattgtcagtttctcaaga

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