GrainGenes Sequence Report: BQ162330

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Sequence

BQ162330

Contig

NSFT03P2_Contig10163

External Databases

TIGR Gene Index

NCBI UniGene

DB Remark

Locus Source: Triticum aestivum (bread wheat)

UniGene title 'Transcribed locus'

Keyword

EST

Species

Triticum aestivum

Cultivar

Chinese Spring

Clone Library

Wheat heat-stressed seedling cDNA library

Tissue

Leaf

Developmental Stage

Two-week old seedling

Data Source

genbank	Release 135, Apr 15 2003
genbank	Updated Nov 2006

Title

WHE0765_F09_L17ZT Wheat heat-stressed seedling cDNA library Triticum aestivum cDNA clone WHE0765_F09_L17, mRNA sequence.

Strain

lab_host E. coli SOLR

Clone

WHE0765_F09_L17

Remark

DB_xref: taxon:4565

Feature: source: mol_type = 'mRNA';

Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer.

Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Two-week old seedling were heat treated at 37 C for 2 hr and cyclic heat treated at 37 C for 2 hr for two days at Texas Tech University (D. Zhang, H. Nguyen lab). The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

DNA

tttttttttaattttttttgttttcttttttttttgaaaaaaaaatgatt
ttcttttctagatatggactttgtacacagagggagcttatgtcatatgt
tacaaaacccctgcctactttagcaatactgatcctacaggggcatatca
agcagcgcacgtttgcagct


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