GrainGenes Sequence Report: Ta.8767.1.S1_a

GrainGenes Sequence Report: Ta.8767.1.S1_a_at

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Sequence

BQ162346

Contig

Ta.8767.1.S1_a_at

Ta.8767.2.A1_at

External Databases

TIGR Gene Index

wEST map position

BQ162346

NCBI UniGene

Ta.8767

DB Remark

Locus Source: Triticum aestivum (bread wheat)

UniGene title 'Transcribed locus, moderately similar to NP_001050295.1 Os03g0395900 [Oryza sativa (japonica cultivar-group)]'

Keyword

EST

Species

Triticum aestivum

Cultivar

Chinese Spring

Chromosome

4AL

4BL

4DS

Clone Library

Wheat heat-stressed seedling cDNA library

Tissue

Leaf

Developmental Stage

Two-week old seedling

Data Source

genbank	Release 135, Apr 15 2003
genbank	Updated Nov 2006

Title

WHE0766_F11_L22ZT Wheat heat-stressed seedling cDNA library Triticum aestivum cDNA clone WHE0766_F11_L22, mRNA sequence.

Strain

lab_host E. coli SOLR

Clone

WHE0766_F11_L22

Probe

WHE0766_F11_L22

Remark

DB_xref: taxon:4565

Feature: source: mol_type = 'mRNA';

Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer.

Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Two-week old seedling were heat treated at 37 C for 2 hr and cyclic heat treated at 37 C for 2 hr for two days at Texas Tech University (D. Zhang, H. Nguyen lab). The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

DNA

ttttttttttttttttttttttttattttttttttttaacaaaatcaaga
agagagtcgcgcacctcttggaacgatattctgctcataaatatcacaat
cacaaatacactaactctacgctatcaacaatgatgggatccaggacttc
gctgatcggtacataagtaacagggcaccaggaagcccaagtaaataaaa
atgaagcaccacgtgccatagacaaccaatgagctctgagatactttcag
tgccttgatctgcttctgtaccttgaacgagacagtgaaagtgaatggtt
tctt


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